Présentation PowerPoint - ideev - Université Paris-Sud

Transcription

8th IDEEV Day
18th October of 2016
IMAGIF auditorium Gif-sur-Yvette
Site web : <http://www.egce.cnrs-gif.fr/?p=6466>
Contact : [email protected]
8th IDEEV Day
Auditorium IMAGIF, Gif-sur-Yvette CNRS Campus
October the 18th of 2016
Acknowledgements
IDEEV day received the financial support of IDEEV and Paris-Sud University.
Ce document a été compilé et mis en forme à partir des données de la page web d’inscription
via des scripts PHP, Python et LATEX par Jean-Bernard Emond, Sylvie Salamitou.
4
Program
9h30-9h45
Capy Pierre
Introduction
9h45-10h30
Joron Mathieu
The evolution and genomic consequences of adaptive inversion polymorphism in butterflies
Session Complex trait evolution (Chairman : H. Sauquet)
10h30-10h50
Hyacinthe Carole
Evolution of acoustic communication in the blind cavefish Astyanax mexicanus
10h50-11h30
Pause / Posters
11h30-11h50
Ressayre Adrienne
Growth and development in maize plants differing in their flowering time
11h50-12h10
Nadot Sophie
Perianth evolution in Ranunculales
12h10-12h30
Bertrand Loı̈c
Plateforme IPANEMA
12h30-14h
Déjeuner
14h-14h45
O’Malley Maureen
Holobionts, holism, and homeostasis : Does microbiota research really imply any of them ?
6
Session Genetics / Genomics (Chairwoman : C. Dillmann)
14h45-15h05
Timpano Hélène
Single-cell genomics of uncultured eukaryotes : The intriguing case of Metchnikovellids
15h05-15h25
Raffoux Xavier
Diversity of meiotic recombination rate in S. cerevisiae using a high throughput technique
15h25-15h45
Nicolas Stéphane
Unravelling genetic diversity within maize landraces using a DNA pooling method based
on 50K SNP array
15h45-16h05
Courret Cécile
X-chromosome meiotic drive in Drosophila simulans : A QTL approach provides information on the genetic basis of drive suppression
16h05-16h30
Pause / Posters
Session Ecology (Chairman : R. Perronne)
16h30-16h50
Monclùs Raquel
Short and long-term consequences of the early development environment
16h50-17h10
Roturier Samuel
Restoring reindeer lichen pastures after fire in boreal Sweden : an experimental approach
to link indigenous and academic knowledge
17h10-17h30
Wicker-Thomas Claude
Multiple origin of pheromone precursors in Drosophila melanogaster
7
17h30-17h50
Kaiser-Arnauld Laure
Caterpillars betrayed by a digestive enzyme
17h50-18h10
Girondot Marc
A functional model and data for epigenetic of metal contamination in marine turtles
8
Communications
Cécile COURRET
[email protected]
0664424570
EGCE
Bâtiment 13, 1 avenue de la Terrasse
91198 Gif-sur-Yvette
X-chromosome meiotic drive in Drosophila simulans : A QTL approach provides information on the genetic basis of drive suppression.
Meiotic drive is a non-mendelian transmission of chromosomes caused by selfish elements, called segregation distorters, that promote their own transmission into the gametes. In the Paris sex-ratio
system, discovered in Drosophila simulans, segregation distorters located on the X chromosome
prevent the production of Y-bearing sperm, which results in strongly female-biased progeny. Because of the drastic impact of sex-linked distorters on population dynamics and evolution, natural
selection select any element preventing their action. Indeed, in a population where an X-linked
distorter has spread, Y-linked and autosomal suppressors are expected to evolve and restore an
equal sex ratio. This is the case in the Paris sex-ratio system, but nothing is known about those
suppressors, regarding their number and genetic nature. We have used a QTL mapping approach
using recombinant inbred lines (RIL) to characterize the autosomal suppressors. The RILs are
derived from an advanced intercross between two parental lines, one containing suppressors and
the other sensitive to the distorters. The suppression ability of each RIL against the Paris distorter
was determined by appropriate genetic cross. We identified 3 QTL, one on the second chromosome
and two on the third showing a strong epistatic interaction. Furthermore we highlight a strong
selection against suppressor allele(s) on the second chromosome, indicating that the suppressor(s)
could have a deleterious effect on the individual fitness.
10
Marc GIRONDOT
[email protected]
0169157230
ESE
Bâtiment 360, rue du Doyen André Guinier
91400 Orsay
A functional model and data for epigenetic of metal contamination in marine turtles
The epigenetic relate to transmission of character though descendance without the character being
based on structural variation in DNA. There are some evidence for epigenetics for some traits
and some molecular mechanisms have been proposed at cellular level but link between these both
remain elusive.
Olive Ridley marine turtle is a species nesting worldwide with two different strategies : solitary
nesting or arribadas when hundred of thousand females come to nest at the same time in the
same beach. This species is also characterized by extreme asymmetry of the carapace scutes. Metal
contamination in adults of the La Escobilla beach on the Pacific coast of Mexico is studied by
Adriana Cortes for her PhD thesis. Surprisingly, we found a link between adult contamination and
carapace asymmetry whereas carapace asymmetry is fixed during embryological life. Furthermore,
it has been shown in other species that embryo carapace asymmetry is itself dependent on contamination of the egg. Based on the knowledge of metal excretion and metabolism in this species,
we propose an epigenetic mechanism for metal contamination transfert though generations.
11
Carole HYACINTHE
[email protected]
0169824148
DECA Equipe-Neuro-PSI
Bâtiment 32-33, 1 avenue de la Terrasse
Evolution of acoustic communication in the blind cavefish Astyanax mexicanus.
Carole Hyacinthe, Joël Attia, Sylvie Rétaux.
Institut de Neuroscience Paris-Saclay, UMR9197 NeuroPSI, et Université de St Etienne.
Acoustic communication is an essential feature in vertebrates allowing conspecific or interspecific
information exchanges in specific behavioural contexts including social organisation and coordination. Sound production has been demonstrated in fishes during territory defence, courtship and
reproduction suggesting that acoustic communication may play an important role in fish speciation and thus in evolution. However, little is known on how communication evolved in fishes. The
teleost fish Astyanax mexicanus, is a model of choice to tackle the question of evolutionary and
neural bases of behavioural adaptation. This species includes surface fish populations (SF), living
in South and Central America rivers, and several populations of cave-dwelling fishes (CF) inhabiting the permanent darkness of Mexican caves. The blind and depigmented CF present increased
chemosensory and mechanosensory capacities, enhanced oral apparatus as well as modifications in
social, swimming and feeding behaviours, among others.
Here, we asked whether acoustic communication has evolved in cavefish during adaptation to
the subterranean environment, possibly as compensation for the lack of visual communication in
the dark. Acoustic recordings showed that SF and CF do produce sounds. We unexpectedly found
that SF and CF share a large acoustic repertory of seven sound types that includes simple or
complex sounds (i.e. : single clicks and click trains). Quantitative analyses performed after sounds
scoring revealed an overall higher sound production in CF, as well as differences in sound types
preferentially used by each morphotype. Complementing our classical behaviour protocols with
bio-acoustic recording and playback technics, we want to further decipher the significance of sound
production in Astyanax mexicanus to characterize specific acoustic communication contexts and
investigate differences between the two morphotypes.
Moreover, we have performed field recordings in the natural environment of three different caves
in Mexico. Analyses aim at comparing acoustic communication in controlled vs. natural environment, and at investigating the possible existence of acoustic signatures between CF populations
inhabiting different caves.
Work supported by a Lidex NeuroSaclay collaborative grant.
12
Mathieu JORON
[email protected]
0467613203
CEFE
CNRS UMR 5175, 1919 route de Mende
34293 Montpellier
The evolution and genomic consequences of adaptive inversion polymorphism in butterflies
Selection on multiple adaptive traits may favour the suppression of recombination between adaptive
alleles. This may resolve in speciation through ecological divergence. However the maintenance of
complex polymorphisms within populations require a mechanism suppressing recombination between the components of the selected trait and maintaining the adaptive combinations together.
Polymorphic mimicry is a good example and may help understand the evolution of complex traits
more generally. The genetic architecture underlying mimicry polymorphism in the tropical butterfly Heliconius numata has undergone drastic evolution, from a multi-locus architecture to a
supergene behaving as a single Mendelian switch. This locus switches between differentiated suites
of characters inherited together, each combination producing an adaptive, mimetic morphology.
Here I present the evolutionary forces acting on the coadaptation of the different traits, the signatures of their evolution and recombination, and the evolutionary paths leading to the formation
of a polymorphic locus with highly differentiated alleles. Selection for local mimicry is a powerful
force favouring coadaptation, and selection against intermediate phenotypes not matching a mimetic pattern present in the locality has shaped the coordination of dominance between the multiple
genetic elements forming the supergene. Inversions at the supergene reduce recombination and
maintain the coadaptation between variable sites across the entire rearranged chromosomal segment. Association studies of nucleotide variation and phenotypes highlight the multiple genes likely
targeted by selection and kept in linkage disequilibrium within this locus. Finally, phylogenomics
approaches suggest that the initial inversion occurred in a different lineage and that the supergene
was formed by the introgression of an already differentiated allele into a host population. The
implications of this on the mechanisms maintaining polymorphism and on population demography
are discussed.
13
Laure KAISER-ARNAULD
[email protected]
0169823704
EGCE
Caterpillars betrayed by a digestive enzyme / Des chenilles trahies par une enzyme
digestive
The plant-eating insect populations are naturally limited by parasitoids that have evolved very
specific mechanisms to identify, locate and parasitize their host species. The analysis of these mechanisms is important for the use of parasitoids in biological control. Hymenoptera species of the
Cotesia flavipes complex develop into caterpillars which bore into the stems of grasses, including
maize and sorghum. Our work has shown that host recognition and acceptance by the parasitic
wasp were triggered by the perception of proteins present in the oral secretions of the caterpillar.
The aim of the IDEEV project was to perform a proteomic analysis of these oral secretions, and
to check the activity and specificity of a candidate protein, whose identity will be revealed during
the presentation. Calatayud P.-A. et al.
Les populations d’insectes phytophages sont naturellement limitées par des insectes parasitoı̈des,
qui ont développé au cours d’une co-évolution avec leurs hôtes des mécanismes très spécifiques
pour les identifier, les localiser et s’y reproduire. L’analyse de ces mécanismes est importante pour
l’utilisation des parasitoı̈des en lutte biologique. Les espèces Hyménoptères du complexe Cotesia
flavipes se développent dans les chenilles qui forent les tiges de graminées, notamment du maı̈s
et sorgho. Nos travaux ont montré que la reconnaissance et l’attaque de la chenille par la guêpe
parasite étaient déclenchées par la perception de protéines présentes dans des sécrétions orales de
la chenille. L’objectif du projet IDEEV a été d’une part de réaliser une analyse protéomique de
ces sécrétions orales, et d’autre part de vérifier l’activité et la spécificité d’une protéine candidate,
dont l’identité sera révélée pendant l’exposé. Calatayud P.-A. et al.
14
Raquel MONCLúS
[email protected]
0682513968
ESE
91405 Orsay
Short and long-term consequences of the early development environment
The early developmental period is very sensitive to disturbances, and parents are known to modify
it through physiological and behavioural modifications. In this project, I am going to investigate
the short- and long-term consequences of the early environment in development and fitness of
chicks. For that, I will consider female promiscuity as a modifier of the early environment because
(a) promiscuous females are more exploratory, more active, and thus, less nest-attending, and (b)
males paired with promiscuous females decrease their paternal input. Therefore, offspring raised
under such conditions are expected to reach independence with a compromised body condition,
decreasing their chances to survive until reproductive age. Moreover, I will explore whether females
are able to compensate for the losses of paternal effort by increasing their own breeding effort, or
whether chicks can compensate for the reductions in parental support, and what are the costs
associated with those strategies. For that I will use spotless starlings (Sturnus unicolor ) breeding
in nest boxes.
15
Sophie NADOT
[email protected]
0169155665
ESE
91405 Orsay
Perianth evolution in Ranunculales
Authors : Sophie Nadot, Hervé Sauquet, Florian Jabbour, Boris Domenech and Catherine Damerval
Angiosperms are characterised by one of the most striking evolutionary novelties of land plants,
the flower, which presents an extraordinary diversity in shape, size and colour in relation with
pollination, a key step in the life cycle of flowering plants. This evolutionary novelty accounts for
the fact that angiosperms are by far the most successful clade of land plants. In most angiosperm
species flowers possess a differentiated perianth composed of sepals and petals, but a significant
number of species displays an undifferentiated perianth composed of a single category of organs,
called tepals. The order Ranunculales is one of the few orders that includes species with either a
differentiated or an undifferentiated perianth. This order is the first diverging order within eudicots
(the largest clade of angiosperms), it is therefore a key group to understand the evolution of the
flower.
Progress has been made towards the elucidation of phylogenetic relationships in the Ranunculales, providing now a relatively robust framework to address the key issue of the ancestral vs.
derived nature of a differentiated perianth within the order as a whole. Here, we present ancestral
state reconstructions for several perianth characters including perianth differentiation, symmetry,
shape of petals and presence/absence of nectaries, with a focus on two major families of the orde,
Ranunculaceae and Papaveraceae. The results are discussed with regard to evo-devo studies focused on identifying genes involved in floral organs identity (the so-called ABC model) and floral
symmetry in Ranunculales.
16
Stéphane NICOLAS
[email protected]
0169332369
GQE - Le Moulon
Ferme du Moulon
Unraveling genetic diversity within maize landraces using a DNA pooling methods
based on 50K SNP array
Maize landraces germplasm have a very large genetic diversity that is still poorly characterized
and exploited in plant breeding programs. We studied the effect of both human selection and environmental adaptation on genome-wide diversity of landraces with a focus on landraces-hybrid
transition in order to identify interesting source of genetic diversity to enlarge modern inbred
lines pool. We developed a high-throughput, cheap and labor saving DNA pooling approach based on 50K SNP maize Illumina array and estimated thereby allelic frequencies of 23412 SNP in
156 landraces representing worldwide maize diversity. We compared diversity at both global and
genome-wide scale level with a diversity panel of 336 inbred lines. Our new approach : (i) gives
accurate allelic frequencies estimation that are reproducible across laboratories, (ii) protects both
against detection of false allele presence within landraces population and against ascertainment
bias. Modified Roger´genetic Distance estimated from 23412 SNP and 17 SSR on same DNA
pool are highly correlated validating our approach. Accordingly, structuration analysis based on
SNP give consistent results with SSR for highly level of structuration but give a slightly different
pictures for more advanced structuration level suggesting that SNP and SSR could capture differently recent evolution. Gene diversity varies strongly globally and along the genome between
the landraces and according to their geographic origins. We identified 376 SNP under diversifying
selection unraveling a selective footprints in Tga1/Su1 regions. While some maize landraces were
closely related to several inbred lines and strongly contributed to modern breeding pool as Reid
Yellow Dent or Lancaster Surecrop, some other have no related inbred lines and seems to have
poorly contributed. Surprisingly, we identified few diversity loss or selective sweep between landraces and inbred lines excepted in centromeric regions. Theses landraces and genomic regions
could be interesting to enlarge genetic diversity of modern breeding pool.
17
Maureen O’MALLEY
[email protected]
0557574633
ALYSAI / CIRID
Université Bordeaux 146 rue Léo Saignat
33076 Bordeaux
Holobionts, holism, and homeostasis : Does microbiota research really imply any of
them ?
Metagenomic methods have made major ecological and evolutionary advances in the last decade.
But as well as enabling the collection of massive bodies of data and many surprising insights into
microbial biodiversity, such methods have also given rise to concepts and expectations about microbiota, particularly the microbiota that live in ongoing symbiotic relationships with large-organism
hosts. The more obvious and entrenched these relationships seem to be, the more language about
mutualism, ‘holobionts’, and homeostasis is being used. Many users of such language assume or
argue that holistic system-wide perspectives are necessary to understand such symbiotic relationships. From this position, many holobiont advocates go on to suggest that as functional wholes,
holobionts must also be evolutionary wholes or multispecies units of selection. Such assertions have
been much contested lately from more standard evolutionary perspectives. Although I will outline
these debates, my focus will be on how holobiont or strong microbiota positions emerged in the
literature. I will suggest that they are methodological artefacts that are produced by the way in
which metagenomic methods developed and were utilized. My talk will conclude with a discussion
of what this means for concepts of holobionts, holism, and homeostasis in microbiota research.
18
Xavier RAFFOUX
[email protected]
0606572690
GQE - Le Moulon
Ferme du Moulon
91190 Gif sur Yvette
Diversity of meiotic recombination rate in S. cerevisiae using a high throughput technique
Meiotic recombination is a major driver of genome dynamics and thus evolution in sexually reproducing organisms. The numbers of crossing-over events and their positions along the chromosomes
are tightly regulated, but the mechanisms involved are still not well understood. Getting more
insights into the regulation of recombination rate and crossover distribution would be beneficial
for many fields of fundamental and applied genetics, in particular to improve the efficiency of
plant breeding. To study the natural genetic diversity of recombination rate, we have set up a high
throughput method to measure crossover rates in S. cerevisiae. We constructed 8 tester strains
carrying three different fluorescent markers at chosen loci, and we used flow cytometry to measure
the segregation of the three markers in the progenies of hybrids between the testers and various
yeast strains. We extracted the recombination rates and the strengths of crossover interference for a
collection of yeast strains representing a large part of the diversity of the species. For a given interval, we observe up to four-fold variations of recombination rate within the collection. Interestingly,
significantly different recombination rates are observed between strains of different geographic origins, but not between strains from different habitats. We also find that most chromosomal regions
exhibit only weak interference.
19
Adrienne RESSAYRE
[email protected]
0169332359
GQE - Le Moulon
Ferme du Moulon
Growth and development in maize plants differing in their flowering time
Flowering time is pivotal in plant life cycle because seed yield depends on the successful meeting
of female and male gametophytes that marks the beginning of the new sporophytic generation. To
flower at a favorable period is therefore crucial for seed production but it is not sufficient to ensure
the completion of the plant life cycle as plants need to produce viable seeds with enough resources
to permit the recruitment of the next generation. Flowering time is therefore a major component
in the adaptation of plants to their environment and an major agronomic trait.
Maize is a plant with a deterministic growth. Its production of vegetative organs ceases with
the floral transition that marks the onset of its reproductive development, fixing its architecture.
A shift in flowering time may therefore has a large impact on many characteristics of the plant.
We have studied the consequences of flowering time shifts in two genotypes issued from thirteen
generations of a divergent selection experiment on flowering time conducted within a maize inbred
line.
Flowering time shift can come either from heterochrony of development or changes in the rate
of organ production or in the rate of organ growth, or a combination of these factors. We followed maize plant growth in the field to monitor leaf production, and plant height until the plants
were sacrificed and dissected to characterize the patterns of growth and organs production and
determine the time of the floral transition. We observed significant changes in the rate of leaf
production as well as in the rate of leaf growth between Early and Late genotypes, indicating that
divergence for flowering time has affected the whole plant´developmental pattern. Nevertheless,
maize development appears remarkably synchronized and we are trying to use the high coordination between organs to try to identify a subsample of traits able to provide a good summary of
maize development. In addition we would like to finely characterize cell division rates in order to
be able to differentiate the components of leaf growth rates.
20
Samuel ROTURIER
[email protected]
0169155670
ESE
91405 Orsay
Restoring reindeer lichen pastures after fire in boreal Sweden : an experimental approach to link indigenous and academic knowledge
For the past 150 years commercial forestry in northern Sweden has greatly modified the fire regimes of the boreal forest to protect timber resources from uncontrolled fires. Fire suppression may
have detrimental effects on the functioning of ecosystems and biodiversity, but also on the reindeer
husbandry practiced by indigenous Sami people who depend on ground lichen for the survival
of their reindeer. The reduction in annual burned area during the last 100 years has resulted in
denser stands, higher productivity of forest soils and more extensive ground vegetation, creating
less favourable habitats for reindeer lichens. Reintroducing burning is an option to restore reindeer
winter pastures in the intermediate to long term. In 2008, a field experiment was established combining fire and reindeer lichen transplantation to gain a better understanding of the ability and the
requirements of lichen thalli to establish on burnt surfaces following different post-fire treatments
and transplantation methods. This was the first large-scale attempt to transplant reindeer lichen
to burnt areas. Eight years after establishment, the results showed a continuous increase in lichen
cover, reaching 75% of area of occupancy by established lichen fragments, on average. Post-fire
treatments, doses and transplantation seasons had significant effects on lichen establishment. The
experiment reveals useful results for the restoration of reindeer lichen and for specifying fire management regimes adapted to different land users. In addition, it initiated a learning process involving
a Sami community and the forest company, which provided common ground for transmitting and
creating new knowledge and management strategies. We believe that there are socio-ecological
aspects associated with the restoration/rehabilitation of fire regime that must be tackled using all
sources of knowledge available, scientific and indigenous and local knowledge.
21
Hélène TIMPANO
[email protected]
0169156342
ESE
91405 Orsay
Single-cell genomics of uncultured eukaryotes : the intriguing case of Metchnikovellids
Single-cell genomics of uncultured eukaryotes : the intriguing case of Metchnikovellids
Hélène Timpano 1 , Elena Nassonova 2 , 3 , David Moreira 1 , Guifre Torruella 1 , Alexey Smirnov 2 and
Purificación López-Garcı́a 1
Microorganisms are extremely diverse, play crucial ecological roles and hold, in their genomes,
the key to our evolutionary history. To reconstruct major diversification events, we need genomic and/or transcriptomic information from a wide range of prokaryotic and eukaryotic lineages
including, notably, divergent lineages that occupy pivotal positions in the tree of life. However,
most microbial diversity escapes cultivation in the laboratory. Single cell-based approaches allow
overcoming this limitation and obtain genome/transcriptome data from individually sorted cells
and extremely low nucleic acid quantities. Single-cell ‘omics’ face several technical challenges, such
as efficiently sorting cells of interest, obtaining high quality nucleic acids or avoiding contamination. We have recently set up the UNICELL facility, funded by the ERC and Region Ile-de-France,
to develop and adapt protocols for single-cell ‘omics’ after high-throughput sorting or individual
cell manipulation. Here, we present a single-based phylogenomic analysis of an intriguing group of
eukaryotes, the metchnikovellids. Metchnikovellids are hyperparasites that infect gregarines parasitizing, in turn, polychaetes. They were first described in 1897 by Caullery and Mesnil who observed
them in the gut of polychaetes collected from a Normandy beach. Their phylogenetic affiliation has
been controversial ever since. We performed whole genome amplification from a metchnikovellidinfected single gregarine cell collected from a polychaete living in the White Sea. We sequenced
this single-cell amplified genome (SAG) using Illumina HiSeq paired-end sequencing. Phylogenomic
analysis revealed that metchnikovellids are the earliest known branch of Microsporidia, and that
they seem to have lost mitochondria and likely all mitochondria-related genes.
1. Unité d’Ecologie, Systématique et Evolution, Université Paris-Sud, Orsay, France
2. Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia
3. Institute of Cytology RAS, St. Petersburg, Russia
22
Claude WICKER-THOMAS
[email protected]
0169823708
EGCE
Multiple origin of pheromone precursors in Drosophila melanogaster
Drosophila pheromones (CHCs) are made in specialized epidermal cells called oenocytes. CHC
biogenesis consists of long-chain fatty acid (LCFA) synthesis, optional desaturation(s), elongation
to very-long chain fatty acids (VLCFAs) and removal of the carboxyl group. We have previously
demonstrated that all the steps following LCFA synthesis occur within the oenocytes. Here we show
that LCFA production depends from a synthesis within the oenocytes and within the fat body and
from food lipids. Food during larval stages is very important for adult pheromone production and
flies that emerged from larvae that were fed with excess dietary lipids had decreased pheromone
amounts, and their mating success was lower. Our study highlights the importance of environmental
and physiological inputs in regulating LCFA synthesis to eventually control sexual communication
in a polyphagous animal.
23
Loı̈c BERTRAND
[email protected]
00169359009
IPANEMA
Synchrotron Soleil
Ipanema platform
.
24
Posters
Adel Amar AMOURI
[email protected]
0213552065834
Laboratoire d’écologie végétale
Département de Biologie. BP 1524. Faculté SNV. Université d’Oran 1 Ahmed Ben Bella.
31000 Oran - Algérie
Phonotypical and Molecular Investigation In Medicago truncatula Gaertn. Ecotypes
under Salt Stress
In our study, we assessed the phenotypic variability of eleven ecotypes of M. truncatula Gaertn.
under salt stress (137 mM NaCl) compared to the control at the germination stage. For the analysis
of seedling growth under salinity stress, it will be useful to study root growth elongation. Several
studies are focalized in root development because it is the most sensitive part of the plant and
controls rapid transmission information to other plant parts. The Results showed that Tru 131 ecotype, with a high ratio (Root more vigorous than shoot) is more tolerant to salinity stress than the
sensitive ecotypes. For the molecular analysis, four expressed sequence tag-simple sequence repeat
EST-SSRs primers (MTIC 044, MTIC 124, MTIC 077 and MTIC 335) were used to show genetic variability in different ecotypes of M. truncatula Gaertn. comparing with the two contrasting
genotypes Tru 131, tolerant genotype and Jemalong, sensitive one. The polymorphic information
content (PIC) ranged from 0.12 to 0.49. These EST-SSRs markers were more polymorphic except
MTIC 044. We have chosen the most polymorphic EST-SSR (MTIC124) in order to determine a
potential link between this marker and salt stress tolerance on the two contrasting genotypes Tru
131and Jemalong. The amplification of the DNA isolated from 10 individual seedlings for each genotype with the EST- SSR (MTIC 124) produced a total of 20 amplified products. The analysis of
polymorphism locus showed that the tolerant genotype Tru 131 population had two alleles, genetic
diversity index of 0.32 and PIC value of 0.267. The results obtained from UniGene and Uniprot
databases of highly similarity proteins sequences with the EST- SSR (MTIC 124), showed that this
locus encode cysteine proteinase inhibitor, and was expressed principally in root in M.truncatula.
This data suggest that this locus is involved in salinity tolerance, and it is appropriate for understanding salt stress tolerance mechanisms in Medicago truncatula Gaertn.
Keywords— Medicago truncatula Gaertn., Molecular databases, Molecular markers, Salt stress
26
Clément MABIRE
[email protected]
0169157623
GQE - Le Moulon
Ferme du Moulon
Genome-wide association study between 60 000 Present/Absent Variants and 29 agronomic traits using a new high throughput genotyping array
Large extent of Copy Number Variants (CNV), i.e changes in the copy number of genes between
individuals, have been recently highlighted in maize using Comparative Genomic Hybridization
array or massive parallel sequencing. However, their contribution to genetic diversity and to traits
variation remains mostly unknown since these technologies are very expensive and labor demanding.
To address these issues, we developed an original approach based on Affymetrix Axiom technology, to genotype an extreme form of CNV called Present Absent Variant (PAV) in maize. PAV
was defined as DNA sequence >1kbp that is present in some individuals but absent from others.
Using our high throughtput genotyping array, we genotyped 60 026 PAV on 356 inbred lines
from an association panel representing worldwide maize genetic diversity. This panel has been previously genotyped using 50k SNP Illumina Infinium array and phenotyped for 29 agronomic traits
related to yield, phenology and plant architecture. We analyzed and compared how PAV and SNP
polymorphisms were globally structured in these panel by analyzing relatedness and genetic structuration. We observed that genetic structuration and relatedness obtained using PAV were globally
similar to those obtained with SNP. We analyzed extent of linkage disequilibrium (LD) between
SNP and PAV. We observed that LD were less extended between PAV and SNP than between
SNP suggesting that the effect of these polymorphisms on traits could be not easily captured by
linkage disequilibrium with SNP from 50K array. We performed a genome wide association study
on 29 agronomic traits and identified several PAV significantly associated with different agronomic
traits.
27
Laure BARTHES
[email protected]
0169155701
ESE
91405 Orsay
PROJET TIP TOP : Tree InPuT OnProgress
Le projet TIPTOP vise à évaluer en simultané la dynamique saisonnière de la fourniture d’azote
par le sol en relation avec les absorptions racinaires printanière. Il s’agit de comprendre comment
les activités microbiennes de minéralisation, et d’immobilisation se comportent à cette phase-clef de
l’absorption racinaire. En effet, il n’existe que peu d’études qui couplent le fonctionnement interne
de l’arbre à celui du sol, les études étant généralement réalisées indépendamment sur ces deux
compartiments. La stratégie proposée consiste à suivre le devenir d’une solution de15NH415NO3
appliquée sur le sol pour estimer le prélèvement de l’azote minéral par les arbres et la microflore
et évaluer ainsi le rôle de la compétition microbienne dans l’acquisition de l’azote par les arbres au
printemps. .
28
Emmanuelle BAUDRY
[email protected]
ESE
91405 Orsay
Pollination service in periurban areas : the bees of Saclay
.
29
Stéphane BAZOT
[email protected]
0163157136
ESE
91400 Orsay
Rhizospheric soil microbial community and beech decline in Fontainebleau forest
Since the end of 90’s, a new wave of forest decline has been observed in France. So far, few
studies have dealt with the underlying processes of this forest decline. If the visible symptoms
are well characterized, few studies are available on the mechanisms leading to the decline. This
work deals with the characterization of the activity and diversity of microbial communities in the
rhizosphere in a mature beech stand in Fontainebleau forest. The aim is to study the biological
activity of the soil through soil respiration fluxes. The size of microbial biomass is measured
through rates of organic nitrogen and carbon. Community structure is analysed with T-RFLP.
Our measurements are performed before bud break. While growth is strongly affected (Delaporte
et al 2016), there seems to be no real differences in activity and structure of microbial communities
in the rhizosphere between healthy and declining trees. These results will be completed by the
study of microbial density (qPCR). In contrast, a seasonal variation in the size and structure of
microbial communities in the soil is shown, regardless of the state of the tree.
30
Gaelle CLAISSE
[email protected]
0169823721
EGCE
Localization and temporal tracking of the sex-peptide Acp70A involved in the reproduction of female fruit flies
In sexual species, male reproductive success depends on attractiveness of female and receptivity
to mating. These elements are under the control of some genetically determined chemical signals,
acting on the female physiology (decreasing receptivity to other mating and increasing egg laying)
and improving therefore the paternity of male. The seminal fluid proteins produced by the accessory glands (Acps) play a key role in this process, both in vertebrate and invertebrate.
In Drosophila melanogaster, the Acps proteins are well studied and about thirty of them are
functionally characterized. It has been suggested that the duration of inhibition of female sexual
receptivity would depend on the sex-peptide bond time (Acp70A) to sperm in the female sperm
storage organs, also the sperm would act as carrier of Acp70A which would be released over time.
To date, these processes of binding and uncoupling of sex-peptide from the sperm tail are disclosed
in Drosophila melanogaster (Peng et al., 2005).
To study the effective role of Acp70A between different strains of Drosophila melanogaster showing
different mating strategies (monoandrous or polyandrous), we have develop a specific antibody 1)
to accurately locate Acp70A in the female genital tract and 2) to follow his persistence in time.
The results show a transient signal of detection, and stronger in the case of monoandrous, and
detection over time in agreement with sperm as carrier.
31
Natalia CONDE E SILVA
[email protected]
0169153411
GQE - Le Moulon
Ferme du Moulon
Genetic and epigenetic mechanisms controlling NdAP3-3 expression and its relationship with floral dimorphism in the Ranunculaceae Nigella damascena
Conde e Silva N, Deveaux Y, Le Guilloux M, Damerval C
In flowers, the perianth composed of sterile organs serves two ecological functions, namely protecting developing fertile organs and attracting pollinators in order to ensure reproduction. Bipartite
perianths originate from the differentiation of the organs in two morphologically and functionally
distinct structures, the outer protective sepals, and the inner attractive petals. Floral organ identity is defined by the ABCE model and the petal is specified by the expression of B-class genes.
The Ranunculaceae is a remarkable group to analyze the development of petals, because they
include species with a large variety of flower color, size and form. Nigella damascena presents a
rare perianth architecture dimorphism. The [P] morph presents usually five sepals and eight petals.
In contrast in the [T] morph, petals are absent and the perianth is composed of a single type of
sepaloid organs, the tepals. The latter phenotype is caused by the inactivation of a single B-class
gene, the NdAP3-3 gene. In order to characterize the mechanism that inhibits the expression of
NdAP3-3 in [T] morph, the chromatin context and transcriptional activities of each allele were
examined in different genetic backgrounds.
32
Christine DILLMANN
[email protected]
0169332348
GQE - Le Moulon
Ferme du Moulon
The Itemaize project : Integrative Approaches of Flowering Time Variation in Maize
Using maize as a model crop, and building upon complementarities between project partners,
Itemaize addresses two important BASC research questions. First, it will better characterize and
possibly predict the effects of environmental changes on living species, including interactions between organisms. Second, it will study the ability of organisms to adapt to global changes. Bringing
partners from different disciplines, the project relies on a unique plant material resulting from 20
years of divergent selection for flowering time performed in the Plateau de Saclay. Selecting each
year for early and late flowering from a narrow genetic diversity (two inbred lines), we created an
evolved plant material likely to be enriched in (epi)genetic differences related to flowering time,
while preserving the original characteristics of the initial inbred lines. Comparisons among generations allow investigating the dynamics of the response to selection in a changing environment.
Comparisons between Early and Late families allow investigating the genotype-phenotype map.
Early and Late progenitors from generation G18 will be used to perform in-depth characterization of plants growth and development (Task 1). Integration of different scales (from the genetic
level to the whole plant growth dynamics) will make use of both partner´expertise and strong
investment in statistical modelling. Data will serve to calibrate a plant growth model that couples
development, phenology and metabolism (Task 2) to better understand how the environment can
modulate maize life-cyle, as well as to decipher between genetic and plastic bases for life-cycle
shifts. An evaluation trial of all plant material of the selection experiment will help to monitor
and modelize genetic and phenotypic changes that occurred during the response to selection, and
to better understand genotype-phenotype relationships. Again, the project will benefit from both
practical (phenotyping) and theoretical (quantitative and population genetics) advances from the
partners, as well as from a strong input from mathematics. Finally we will use climatic data from
the last 20 years, along with the observed response to selection, to describe links between environment and the dynamics of adaptation. Using Lepidoptera stem borers as a model system, we will
also analyse how plant phenology shifts interfere with pathogen life-cycles. Altogether, Itemaize
will help to (i) better understand how environment impacts plant life-cycles and their interaction
with insect pests, (ii) predict the potential for (epi)genetic adaptations and (iii) define selection
criteria for crop life-cycle shifts. The project will also sustain methodological developments on
phenotyping, data analyses and modelling.
33
Caroline DRACXLER
[email protected]
0641597238
MNHN
1, rue du Petit Château
91800 Brunoy
Vulnerability of Palms in Tropical Forests
34
Tatiana GIRAUD
[email protected]
0169155669
ESE
91400 Orsay
Evolution of sex chromosomes in fungi
Sex chromosomes in plants and animals and fungal mating-type chromosomes often show exceptional genome features, with extensive suppression of homologous recombination and cytological
differentiation between members of the diploid chromosome pair. Despite strong interest in the
genetics of these chromosomes, their large regions of suppressed recombination often are enriched
in transposable elements and therefore can be challenging to assemble. Here we show that the
latest improvements of the PacBio sequencing yield assembly of the whole genome of the anthersmut fungus, Microbotryum lychnidis-dioicae (the pathogenic fungus causing anther-smut disease
of Silene latifolia), into finished chromosomes or chromosome arms, even for the repeat-rich matingtype chromosomes and centromeres. Suppressed recombination of the mating-type chromosomes
is revealed to span nearly 90% of their lengths, with extreme levels of rearrangements, transposable element accumulation, and differentiation between the two mating types. We observed no
correlation between allelic divergence and physical position in the nonrecombining regions of the
mating-type chromosomes. This may result from gene conversion or from rearrangements of ancient
evolutionary strata, i.e., successive steps of suppressed recombination. Centromeres were found to
be composed mainly of copia-like transposable elements and to possess specific minisatellite repeats
identical between the different chromosomes. We also identified subtelomeric motifs. In addition,
extensive signs of degeneration were detected in the nonrecombining regions in the form of transposable element accumulation and of hundreds of gene losses on each mating-type chromosome.
Furthermore, our study highlights the potential of the latest breakthrough PacBio chemistry to
resolve complex genome architectures.
35
Arnaud LE ROUZIC
[email protected]
0169823765
EGCE
EDENS : Evolutionary impact of group density : an experimental evolution approach
Density dependence is the fundamental process through which populations reach their ecological
equilibria. Since fitness of an individual is nothing but a measure of the demography of its offspring,
selective pressures must be influenced by density-dependent processes.
Variation in population density is well known to affect a large spectrum of ecological and evolutionary mechanisms. Increasing population density affects individual resource acquisition in terms
of food, mating or space, but also social interactions and epidemiological processes. On an evolutionary timescale this may impact mating system, food competitive ability, immunological traits. . .
The relationship between population density and evolution of competitive ability has been extensively investigated experimentally, especially when considering energy acquisition and usage.
Experimental populations kept over generations at high density tend to evolve increased competitive ability compared to population kept at low density with the same amount of limited resource.
This increased competitive ability has been repeatedly seen to trade off with other phenotypic
traits such as body size or immunological performance. The evolutionary impact of social
interactions themselves has however been poorly experimentally investigated. Intuitively, social interaction may appear as an important evolutionary driving force of mating systems.
However empirically, there seems to be a perplexing variety of options of how competition for
mates, mate searching, mate choice, sexual conflict and parental care can respond to density. Furthermore simple social interactions may not be neutral and affect the behavior of the individual
(or even its physiology) and thus have evolutionary consequences. How does phenotypic plasticity
affect evolutionary outcomes is unknown. Until now most experimental approaches on the impact
of group density were based on either comparative data or on the measurement of plastic response
to variation in group density. We are still poorly informed on the impact of social interaction in
the evolution of phenotypic (morphological, physiological, or behavioral) traits.
We believe that experimental evolution is a powerful research tool to study the evolutionary impact
of group density. EDENS propose to study the evolutionary impact of variation in density of adult
group of Drosophila melanogaster on a wide array of phenotypic traits. The aim of this starting
project is to initiate a long-term experimental evolution which will serve as a collaborative tool for
phenotypic and genetic measures.
36
judith LEGRAND
[email protected]
0169332349
GQE - Le Moulon
Ferme du Moulon
Interspecific interactions in wheat sourdough microbial communities
Microbial communities are ubiquitous across earth ecosystems. Thanks to metagenomic methods,
new data is gathered every day about their species composition. However very little is known about
the ecological processes governing their patterns of biodiversity and how the species forming these
consortia interact together.
Wheat sourdough’s microbial communities are relatively simple communities which can be a useful model to address these questions. Sourdough communities are composed of an assemblage of
lactic acid bacteria and yeasts of which species composition vary between sourdoughs. It has been
hypothesized that yeasts and LAB interact together mainly in a non-competitive way through
mutualistic supply of important metabolites, such as glucose release by LAB, or vitamins release
by yeasts to the benefits of each other. However interspecific interactions in sourdough have not
been extensively studied. Consequently, the validity and generality of these hypotheses, as well as
the nature of interactions between yeasts or between LABs species remains to be tested.
In this study, we have selected different communities sampled from farmer-bakers´ organic sourdoughs. Then we used co-culture experiments to characterize the nature of interactions (competition, facilitation,. . . ) between pairs of species (LAB/Yeast). We have shown that LAB presence
tends to decrease yeast density whereas yeast presence tends to decrease LAB density. However,
results are contrasted depending on species and strains.
We are currently using numerical simulations to test the hypothesis that species interactions are
the net results of multiple mechanisms and to assess the minimal “mechanistic complexity” needed
to explain the observed pattern.
37
Frédéric MARION-POLL
[email protected]
0169823756
EGCE
Does aerial pollution affect insects ?
Air quality is a major concern to human populations living in urban cities such as Paris, New Delhi
or Beijing. It has been identified as a major health issue, as it is made responsible for increasing
levels of allergies, asthma, stress and even for reducing the life span of individuals regularly exposed
to it. This pollution has many causes, but the nano-particles emitted by diesel vehicles and wood
combustion are among the most dangerous. While this legitimate concern is growing, no one has
looked at the impact of such particles on insects. Given the size of these particles which can easily
penetrate trachea, given their biological activity on cells in culture, and considering their strong
smell (or taste), one would expect such particles to have a strong impact on insects which are good
flyers such as pollinators. We will examine these different hypotheses and present preliminary data
collected in a heavily polluted city of India, Bangalore, concerning hoverflies, in order to discuss
the possibility to study the impact of airborne pollution on insects and establish a network of
similar observations in the Paris area.
38
Rémi PERRONNE
[email protected]
0169332345
GQE - Le Moulon
Ferme du Moulon
Influence of yellow rust epidemics over the commercial life cycle of bread wheat varieties between 1985 and 2004
Rémi PERRONNE 1 , Solène DIGUET 1 , Claude DE VALLAVIEILLE-POPE 2 , Marc LECONTE 2 ,
Jérôme ENJALBERT 1
Yellow rust caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases
of bread wheat in France. The breakdown of resistance genes by the fungal populations sometimes
leads to the rapid withdrawal of highly susceptible varieties from the market, but no study has so
far described the commercial life cycle of varieties of arable crops. In this study, we proposed a set
of indicators of varying complexity to study how pathogen pressures influence these commercial
life cycle of varieties that we described for around 50 varieties sown on at least 10,000 hectares
from 1980 to 2010. The commercial cycle of varieties was studied for four distinct epidemics within homogeneous agricultural regions in terms of yellow rust pressure, yellow rust pathotypes and
landscape varietal composition. Based on principal components analyses and clustering methods,
a set of four indicators allowed identifying varieties with distinct commercial life cycle, i.e. leading varieties, varieties with a long commercial life cycle and varieties with a rapid withdrawal
potentially influenced by a yellow rust epidemic. The distinction between susceptible varieties and
varieties with a durable resistance against yellow rust was confirmed based on the characterization
of virulence profiles of pathotypes and postulation of resistance genes of the varieties. We also highlighted that the epidemic context has a strong influence on the number of varieties characterized
by a rapid withdrawal from the market.
1. UMR GQE – Le Moulon, INRA, Univ Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91190,
Gif-sur-Yvette, France, [email protected]
2. UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France
39
Rémi PERRONNE
[email protected]
0169332345
GQE - Le Moulon
Ferme du Moulon
Using a partitioning procedure based on Rao quadratic entropy index to analyze the
temporal evolution of in situ varietal and genetic diversity : the case of bread wheat
in France over the period 1981-2006
Rémi PERRONNE 1 , Isabelle GOLDRINGER 1
The temporal evolution of crop diversity over the second half of the twentieth century has been
analyzed in numerous studies based on various statistical approaches depending on the nature of
the data available. Different statistics have been proposed among which Nei´gene diversity H and
the genetic similarity GS were the most used. These statistics are complementary because Nei´gene
diversity informs on the level of crop diversity observed at a given time while the genetic similarity
informs on the differences in allelic composition among varieties without accounting for acreage
frequencies of varieties. However, to assess the actual genetic vulnerability of crops at landscape
scale, for instance to face with climate instability and pest pressures, considering in situ genetic
diversity integrating acreage frequencies of varieties seems necessary. Until now, no consistent mathematical framework has been proposed for this purpose. Based on a partitioning methodology of
the Rao quadratic entropy index previously applied on community ecology, and datasets characterizing the acreage of varieties and molecular data using microsatellite markers, we first highlighted
that the various components of the temporal evolution of wheat diversity showed distinct patterns.
For instance, at national scale, when studying the allelic composition of years spaced by five years,
we observed an increase in the genetic similarity between varieties sown on years spaced by five
years while we highlighted a clear increase of the genetic distance among varieties sown on years
spaced by five years when integrating acreage frequencies of varieties, especially between 1993 and
1995. This could be due to the predominance of the variety Soissons during this period, a variety
representing more than 30% of the bread wheat acreage at national scale and appearing genetically
distant to most of the other leading varieties over the whole period studied.
1. UMR GQE – Le Moulon, INRA, Univ Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91190,
Gif-sur-Yvette, France
40
Zhang QIAN
[email protected]
0751097264
ESE
91405 Orsay
Estimating divergence times and ancestral breeding systems in Ficus and Moraceae
There are two breeding systems in Ficus (monoecy and gynodioecy). Monoecy has been suggested
to be ancestral in Ficus, with one origin of gynodioecy and at least one reversal to monoecy.
Although several hypotheses have been proposed for the origin of gynodioecy in Ficus, the evolution
of breeding systems in the genus is still incompletely understood. Here, we reconstruct ancestral
states of breeding systems in Ficus and Moraceae as a whole using parsimony, maximum likelihood
and Bayesian approaches. To do so, we first revised the timescale of Moraceae diversification using
new family-wide phylogenetic analyses calibrated with a revised set of 10 internal and outgroup
fossil calibrations. We find that ancestral states for breeding systems in Moraceae in general,
and the ancestral breeding system for Ficus in particular are especially sensitive to : 1) models
of morphological evolution, 2) phylogenetic and dating uncertainty, and 3) taxonomic sampling
(e.g., whether outgroups of Ficus are included or not). For instance, when considering Ficus only
(without outgroups), ancestral monoecy is inferred with parsimony and the equal-rates (ER/Mk1)
model in maximum likelihood (P=0.96), and dioecy with the unequal-rates (ARD/Mk2) model
(P=0.87). Several possible scenarios for breeding system evolution in Ficus emerge including : 1)
ancestral monoecy with one transition to gynodioecy and four reversals to monoecy, 2) ancestral
gynodioecy with seven transitions to monoecy. These results are a first step towards investigating
the relationship among breeding system evolution, geographic events and historical climate change
in Ficus and Moraceae.
41
Hervé SAUQUET
[email protected]
0169154991
ESE
91405 Orsay
Teaching botany to the broadest audience : challenges and success of the MOOC Botanique
On September 5, 2016, the MOOC (Massive Open Online Course)
“Botanique : Apprendre à connaı̂tre les plantes – initiation” opened with a staggering 20,000 registered participants (http ://mooc.tela-botanica.org/). A MOOC is a special kind of online course,
typically including short videos, documents, online quizzes, and forums distributed over a set period, with a start date and an end date. The MOOC Botanique was created and coordinated by the
French association Tela Botanica, which maintains the largest network of francophone amateur and
professional botanists. The project was funded through a Programme d’Investissement d’Avenir
to the consortium Floris’Tic and a grant from IDEEV. Fourteen experts from diverse backgrounds
and French-speaking regions (France, Algeria, Cameroon, Lebanon, New Caledonia, Québec, and
Senegal) share their knowledge and passion for plant biodiversity through 24 videos shot 100%
outdoors by a professional film crew. This MOOC, distributed over a period of seven weeks, is
entirely free and was prepared to target the broadest possible audience by offering an attractive
course on introductory botany and plant identification, without any prior knowledge required. As
the academic director and two of the lead teachers/actors of the project, we here share our experience working on this highly motivating, one-of-a-kind initiative. We will first present the teaser
of the MOOC, which has been used since April 2016 to communicate on the project and attract
participants. We will then briefly outline the history of the project, focusing on some important
challenges such as defining the contents, writing the scripts, and shooting the videos. Last, we will
present the English version of one of the videos from the MOOC (Plant evolution), which was
translated and produced thanks to financial support from IDEEV.
42
Gaëlle VAN FRANK
[email protected]
0169154219
GQE - Le Moulon
Ferme du Moulon
Creation and selection of wheat mixtures adapted to the diversity of practices in organic agriculture
(Poster presented by Gaëlle van Frank and Emma Forst)
Agriculture is facing major challenges nowadays, such as the increase in the environmental stochasticity and the need to reduce input use. Agroecology is a way of answering these issues, promoting
a more sustainable production, for example through organic agriculture (OA). Most varieties are
not adapted to OA´conditions (no chemical inputs to stabilize the environmental variations, high
field heterogeneity). Increasing genetic diversity in fields, for example by mixing varieties, is an
interesting lever since it permits the stabilization of production, the optimization of resources use
and pest and disease control. Farmers usually assemble mixtures with the varieties that perform
best on their farm but mixtures performances is not necessarily correlated with performances in
pure stand. Additionally, mixtures are often chosen on the only criterion of complementary diseases
resistances. Methodological questions also arise regarding the adaptation of selection practices and
criterions.
However, little is known about other criterions to use when assembling varieties in a mixture,
and how to select mixtures, especially on farm.
The DEAP team (INRA GQE - Le Moulon) is working on understanding how varieties should
be assembled in a mixture to take advantage of plant interactions and how to select on-farm mixtures adapted to farmers´ practices and local conditions. Here we present the methods that we use
to this purpose : participatory ideotyping and on-farm breeding.
43
44
Participants
Communications & Posters
Zeineb ACHOUR
[email protected]
0169332372
GQE - Le Moulon
Ferme du Moulon
Emmanuelle BAUDRY
[email protected]
ESE
91405 Orsay
Beatrice ALBERT
[email protected]
0169156529
ESE
91405 Orsay
Stéphane BAZOT
[email protected]
0163157136
ESE
91400 Orsay
Adel Amar AMOURI
[email protected]
0213552065834
Laboratoire d’écologie végétale
Département de Biologie. BP 1524. Faculté SNV.
Université d’Oran 1 Ahmed Ben Bella.
31000 Oran - Algérie
Sylvie AULARD-WIDEMANN
[email protected]
0169823735
EGCE
Laure BARTHES
[email protected]
0169155701
ESE
91405 Orsay
Arnaud BECHELER
[email protected]
0615565175
EGCE
Romain BENOIST
[email protected]
0601370444
EGCE
Paola BERTOLINO
[email protected]
0169156342
ESE
91405 Orsay
46
Mélisande BLEIN-NICOLAS
[email protected]
0169156806
GQE - Le Moulon
Ferme du Moulon
Monique BOLOTIN-FUKUHARA
[email protected]
0169156201
GQE - Le Moulon
Bâtiment 400, rue Gregor Mendel
91405 Orsay
Elsa BONNAUD
[email protected]
0169157961
ESE
91405 Orsay
Pierre CAPY
[email protected]
0169823709
EGCE
Fantin CARPENTIER
[email protected]
0786016759
ESE
91405 Orsay
Yves CARTON
[email protected]
0169823711
EGCE
François CHIRON
Antoine BRANCA
[email protected]
0169154991
ESE
91405 Orsay
Jean-Tristan BRANDENBURG
[email protected]
0683464971
EGCE
[email protected]
0169155687
ESE
91405 Orsay
Hanna CHOLE
[email protected]
0680005542
EGCE
47
Gaelle CLAISSE
[email protected]
0169823721
EGCE
Natalia CONDE E SILVA
[email protected]
0169153411
GQE - Le Moulon
Ferme du Moulon
Adriana CORTéS
[email protected]
0783738312
ESE
91405 Orsay
Jean-Luc DA LAGE
[email protected]
0169823727
EGCE
Jean DAVID
[email protected]
0673882476
EGCE
Gwendoline DAVID
[email protected]
0682044739
ESE
91405 Orsay
Dominique DE VIENNE
Franck COURCHAMP
[email protected]
0169155685
ESE
91405 Orsay
Cécile COURRET
[email protected]
0664424570
EGCE
[email protected]
0169332360
GQE - Le Moulon
Ferme du Moulon
James DESAEGHER
[email protected]
0616863655
ESE
91400 Orsay
48
Christine DILLMANN
[email protected]
0169332348
GQE - Le Moulon
Ferme du Moulon
Sandrine FONTAINE
[email protected]
0169155701
ESE
91400 Orsay
Emma FORST
Caroline DRACXLER
[email protected]
0641597238
MNHN
1, rue du Petit Château
91800 Brunoy
[email protected]
0169332347
GQE - Le Moulon
Ferme du Moulon
Timothée FOUQUERAY
Michael DUBOW
[email protected]
0169154612
I2BC
Bâtiment 409
91405 Orsay
Jean-Bernard EMOND
[email protected]
0169823726
EGCE
Shanerin FALAB
[email protected]
0781881385
ESE
91405 Orsay
[email protected]
0102030405
ESE
91405 Orsay
Nathalie FRASCARIA-LACOSTE
[email protected]
0169155668
ESE
91405 Orsay
Julien FUMEY
[email protected]
0169823759
EGCE
49
Anne GENISSEL
Aurélie HUA-VAN
[email protected]
0130815242
BIOGER
Avenue Lucien Bretignieres
78850 Thiverval Grignon
[email protected]
0169823724
EGCE
Pierre GéRARD
Carole HYACINTHE
[email protected]
0169332361
GQE - Le Moulon
Ferme du Moulon
Tatiana GIRAUD
[email protected]
0169824148
DECA Equipe-Neuro-PSI
Bâtiment 32-33, 1 avenue de la Terrasse
Ludwig JARDILLIER
[email protected]
0169155669
ESE
91400 Orsay
[email protected]
0169155084
ESE
91405 Orsay
Marc GIRONDOT
Rémi JEANNETTE
[email protected]
0169157230
ESE
91400 Orsay
[email protected]
0169823748
EGCE
Christophe HANOT
Dominique JOLY
[email protected]
0169155689
ESE
91405 Orsay
[email protected]
0169823734
EGCE
50
Mathieu JORON
Arnaud LE ROUZIC
[email protected]
0467613203
CEFE
CNRS UMR 5175, 1919 route de Mende
34293 Montpellier
[email protected]
0169823765
EGCE
Juergen KROYMANN
Jane LECOMTE
[email protected]
0169157657
ESE
91400 Orsay
[email protected]
0169155667
ESE
91400 Orsay
judith LEGRAND
Olivier LANGELLA
[email protected]
0169332368
GQE - Le Moulon
Ferme du Moulon
[email protected]
0169332349
GQE - Le Moulon
Ferme du Moulon
Gwendal LATOUCHE
Yves LEVI
[email protected]
0146835366
ESE
Fac Pharmacie
92290 Chatenay-Malabry
[email protected]
0169155695
ESE
91405 Orsay
Philippe LE GALL
[email protected]
0169823750
EGCE
Puri LOPEZ-GARCIA
[email protected]
0165157608
ESE
91400 Orsay
51
Clément MABIRE
[email protected]
0169157623
GQE - Le Moulon
Ferme du Moulon
Natalia MARTINEZ
[email protected]
0602717421
GQE - Le Moulon
Ferme du Moulon
Raquel MONCLúS
Domenica MANICACCI
[email protected]
0169332361
GQE - Le Moulon
Ferme du Moulon
[email protected]
0682513968
ESE
91405 Orsay
Elodie MARCHADIER
Catherine MONTCHAMP-MOREAU
[email protected]
0671829744
GQE - Le Moulon
Ferme du Moulon
[email protected]
0169823720
EGCE
Frédéric MARION-POLL
David MOREIRA
[email protected]
0169823756
EGCE
[email protected]
0169157608
ESE
91405 Orsay
Olivier MARTIN
Florence MOUGEL
[email protected]
0169332336
GQE - Le Moulon
Ferme du Moulon
[email protected]
0169823719
EGCE
52
Sophie NADOT
[email protected]
0169155665
ESE
91405 Orsay
Rose NDEMAH
[email protected]
0656766640
EGCE
Jean De Matha NDENGUé
[email protected]
0755003186
EGCE
Maureen O’MALLEY
[email protected]
0557574633
ALYSAI / CIRID
Université Bordeaux 146 rue Léo Saignat
33076 Bordeaux
David OGEREAU
[email protected]
0169823739
EGCE
Sébastien OLLIER
[email protected]
0169155670
ESE
91405 Orsay
Rémi PERRONNE
Stéphane NICOLAS
[email protected]
0169332369
GQE - Le Moulon
Ferme du Moulon
[email protected]
0169332345
GQE - Le Moulon
Ferme du Moulon
Rémi PERRONNE
Véronique NORMAND
[email protected]
0169156730
ESE
91405 Orsay
[email protected]
0169332345
GQE - Le Moulon
Ferme du Moulon
53
Marianyela Sabina PETRIZZELLI
Sylvie RETAUX
[email protected]
0698523846
GQE - Le Moulon
Ferme du Moulon
[email protected]
0169823452
NeuroPSI
UMR9197, Bâtiment 32, 1 avenue de la Terrasse
Nicolas POLLET
Emilie ROBILLARD
[email protected]
0169823710
EGCE
[email protected]
0169823707
EGCE
Zhang QIAN
[email protected]
0751097264
ESE
91405 Orsay
Xavier RAFFOUX
Samuel ROTURIER
[email protected]
0169155670
ESE
91405 Orsay
Estelle RUNNEBURGER
[email protected]
0606572690
GQE - Le Moulon
Ferme du Moulon
[email protected]
0169823715
EGCE
Adrienne RESSAYRE
Aurélien SAGHAı̈
[email protected]
0169332359
GQE - Le Moulon
Ferme du Moulon
[email protected]
0123456789
ESE
91405 Orsay
54
Sylvie SALAMITOU
[email protected]
0169823743
EGCE
Maud TENAILLON
[email protected]
0169332334
GQE - Le Moulon
Ferme du Moulon
Hélène TIMPANO
Hervé SAUQUET
[email protected]
0169154991
ESE
91405 Orsay
[email protected]
0169156342
ESE
91405 Orsay
Gaëlle VAN FRANK
Jean-François SILVAIN
[email protected]
016983738
EGCE
Alodie SNIRC
[email protected]
0169157049
ESE
91405 Orsay
Najat TAKVORIAN
[email protected]
0169156728
ESE
91405 Orsay
[email protected]
0169154219
GQE - Le Moulon
Ferme du Moulon
Gilles VERGNAUD
[email protected]
0169156208
I2BC
Bâtiment 400, rue Gregor Mendel
91405 Orsay
Clémentine VITTE
[email protected]
0169332357
GQE - Le Moulon
Ferme du Moulon
55
Claude WICKER-THOMAS
[email protected]
0169823708
EGCE
Sara BRANCO
Luis Javier GALINDO GONZALEZ
[email protected]
034649710100
ESE
91405 Orsay
Guillaume REBOUL
[email protected]
0783511623
ESE
91405 Orsay
[email protected]
0637313796
ESE
91405 Orsay
Delphine STEINBACH
Loı̈c BERTRAND
[email protected]
0
GQE - Le Moulon
Ferme du Moulon
[email protected]
0
IPANEMA
Synchrotron Soleil
Guiffré TORRUELLA
Taiadjana FORTUNA
[email protected]
0169157608
ESE
91405 Orsay
[email protected]
0169157049
ESE
91405 Orsay
Jacqui SHYKOFF
Jodie BOLILLA
[email protected]
0169155666
ESE
91405 Orsay
[email protected]
0622799696
ESE
91405 Orsay
56
Ricardo RODRIGUEZ DE LA VEGA
[email protected]
0
ESE
91405 Orsay
Kévin FRADIN
0
EGCE
Jean-Christophe SANDOZ
Ana GUTIERREZ PRECIADO
[email protected]
0642885854
ESE
91405 Orsay
[email protected]
0169823751
EGCE
Ying Chu LO
[email protected]
0
ESE
91405 Orsay
Rafaël PONCE
[email protected]
0
ESE
91405 Orsay
Jérôme ENJALBERT
[email protected]
0
GQE - Le Moulon
Ferme du Moulon
57

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