Immuno/DNA Washer 20X

PI 0150, Rev. A
DCN: 2248 Effective Date: 05/03/2016
IVD
For In Vitro Diagnostic Use
Immuno/DNA Washer 20X
Intended Use
For In Vitro Diagnostic Use.
Summary And
Explanation
20X Immuno/DNA Washer is a concentrated wash buffer used for Immunohistochemistry and in situ
Hybridization procedures. It can be used in autostainers, capillary action slides as well as manual
staining procedures.
Presentation
20X Immuno/DNA Washer contains a buffer solution, Tween 20 detergent and a non-sodium azide
anti-microbial. It is provided in liquid form.
Availability
Catalog No.
BSB 0149
BSB 0150
Storage Store at room temperature
Protocol
Suggested protocol on reverse
Volume
20X
20X
Volume
200 mL
1000 mL
Stability 3 years
Preparation of Working Solutions
For working solution, dilute the concentrated 20X Immuno/DNA Washer with distilled or deionized water 1:20 (50 mL of
the 20X Immuno/DNA Washer diluted with distilled or deionized water to make up 1 L of 1X working solution).
Recommended Protocol
Use the 1X working solution during any step that requires rinsing of slides.
Abbreviated Immunohistochemical Protocol
Step
Peroxidase/AP Blocker
Primary Antibody
1st Step Detection
2nd Step Detection
Substrate-Chromogen
Counterstain
Precautions
ImmunoDetector AP/HRP
5 minutes
30 - 60 minutes
10 minutes
10 minutes
5 - 10 minutes
Varies
PolyDetector AP/HRP
5 minutes
30 - 60 minutes
30 - 45 minutes
Not Applicable
5 - 10 minutes
Varies
Polydetector Plus HRP
5 minutes
30 - 60 minutes
15 minutes
15 minutes
5 - 10 minutes
Varies
Avoid contact with eyes. Flush with large quantities of water if contact occurs. For professional users.
Minimize microbial contamination of reagents. Proper handling procedures should be used. For
complete recommendations for handling biological specimens please refer to the CDC document,
“Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories” (1).
References
1. U.S. Department of Health and Human Services: Centers for Disease Control and Prevention. Guidelines for Safe
Work Practices in Human and Animal Medical Diagnostic Laboratories. Supplement / Vol. 61, January 6, 2012.
Symbol Key / Légende des symboles/Erläuterung der Symbole
In Vitro Diagnostic Medical Device
Dispositif médical de diagnostic in vitro
In-Vitro-Diagnostikum
Storage Temperature
Limites de température
Zulassiger temperaturbereich
Manufacturer
Fabricant
Hersteller
Read Instructions for Use
Consulter les instructions d’utilisation
Gebrauchsanweisung beachten
Expiration Date
Utiliser jusque
Verwendbar bis
Bio SB, Inc. • 69 Santa Felicia Dr., Santa Barbara, CA 93117, USA
Tel. (805) 692-2768 • Tel. (800) 561-1145 • Fax. (805) 692-2769
• E-mail: [email protected] • Website: www.biosb.com
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