The 6th Oil-spill studies and ISDAMP Final conference 11

The 6th Oil-spill studies and ISDAMP Final conference 11

Multibiomarker approach under chronic & acute pollution
conditions : a realistic measure of environmental quality
of the littoral Atlantic coast & the La Rochelle Marina
Marine Breitwieser, Angélique Fontanaud, Valérie Huet, Hélène Thomas-Guyon
© Julien Chauvet – Ville de La Rochelle
The 6th Oil-spill studies and ISDAMP Final
conference 11 March 2015
Coastals areas
 Environment of land-ocean interface
Hydrosystem communication
(watershed soil
nearshores)
 Abundant and diverse marine communities
Scallops, mussels, oyters…
 Impacted by water contamination
Environmental pollutions
 In the littoral zones
Source: Julien Chauvet
 Contamination
Oil pollution
PCB
Phytosanitaries…
Heavy metals
Source: http://www.rtflash.fr
Source: http://www.growswitch.com
 Chronic pollution
Source: http://www.futura-sciences.com/
RISPECT
project (1)
Chronic pollution conditions
Evaluation of the quality of aquatic environment
Mimachlamys varia
Approach of multi-biomarkers
Study of the impacts of the environment
PEPS RISPECT (RISques d'une contamination chimique chronique et conséquences chez le
bivalve PECTinidae Chlamys varia en milieu côtier)
Correlations biomarkers
/pollutants
RISPECT
project (2)
 Four sites Pertuis Charentais1-3
-
“Reference” site : Loix en Ré
-
Water-treatment plant & Harhour : Port Neuf
-
Marina & Expulsion of dred : Les Minimes
-
Charente estuary : Les Palles
N
Loix
Port-Neuf
Les Minimes
10 km
Chlamys varia,
http://www.joc123.webspace.virginmedia.
com
1-2: Bustamante and Miramand, 2004, 2005, 3: Milinkovitch et al., 2015
Les Palles
RISPECT Results
(3)
Biological parameters1 : Enzymes (3) & Metabolite (1)
•
LDH – Laccase :
(+++) Charente estuary
•
GST - MDA :
(+++ ) Marina
Health status of organisms is affected
Modulation of Biological parameters (3 sites)
•
LDH, Laccase  Charente estuary (Pesticides)
•
GST MDA  Harbour area (Heavy metal & Oil pollution)
1: T.Milinkovitch et al., 2015
RISPECT Results (4)
Correlations Biomarkers/Contaminants
GST
LDH
Po-Lac
Ag
As
Cd
Co
Cr
Cu
Fe
Mn
Ni
-
-
-
-
-
-
<0,1
<0,05
-
<0,01
<0,05
-
-
<0,05
-
-
Pb
Se
Sn
V
Zn
-
<0,01
<0,1
-
-
<0,01
<0,1
<0,05
-
-
<0,1
-
-
-
-
-
-
-
-
-
-
-
-
-
<0,05
<0,05
<0,1
-
-
-
-
-
-
-
-
-
<0,05
-
-
-
Inorganic
<0,05 significative corrélation pvalue <0,05
<0,1 significative corrélation pvalue <0,1
Oil pollution and
polychlorobiphenyles
MDA
Pesticides
MDA GST LDH
hexachlorobenzene
<0,05
aldrine
metolachlore
isodrine
24dde
44dde
dieldrine
endrine
naphtalene
benzothiophene
acenaphtylene
<0,1
acenaphtene
<0,1
fluorene
<0,1
Phenanthrene
<0,1
anthracene
<0,1
fluoranthene
<0,1
pyrene
benzoaanthracene
chrysene
benzobfluoranthene
benzoepyrene
benzoapyrene
perylene
PCB153
PCB138
PCB180
Po-Lac
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
<0,05
-
-
-
-
-
-
-
-
-
-
<0,1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
<0,1
-
-
-
Organic
-
-
-
-
<0,1
-
-
<0,1
-
-
-
-
-
<0,05
-
<0,1
<0,05
-
-
RISPECT Results (4)
MDA
GST
LDH
Lac
Ag
-
-
-
-
As
-
-
<0,1
<0,05
Cd
-
<0,01
<0,05
-
Co
-
<0,05
-
-
Cr
-
<0,01
<0,1
-
Cu
-
<0,01
<0,1
<0,05
Fe
-
-
<0,1
-
Mn
-
-
-
-
Ni
-
-
-
-
Pb
-
-
-
-
Se
-
<0,05
<0,05
<0,1
Sn
-
-
-
-
V
-
-
-
-
Zn
-
<0,05
-
Inorganic
-
<0,05 : significative corrélation pvalue <0,05
<0,1 : significative corrélation pvalue<0,1
Enzymes (3)
GST MDA Lac
Correlations
Biomarkers/ INORGANIC
Contaminants
Heavy metals
Cd Co Cr
Cu Se Zn
RISPECT Results (4)
Correlations
Biomarkers /ORGANIC
Contaminants
HAP Pesticide
PCB…
mareesnoires.e-monsite.com
Scallop
Environemental Sentinel Specie
<0,05 : significative corrélation pvalue <0,05
<0,1 : significative corrélation pvalue <0,1
Oil pollution
Biological parameters (4)
Pesticides
MDA GST LDH
hexachlorobenzene
<0,05
aldrine
metolachlore
isodrine
24dde
44dde
dieldrine
endrine
naphtalene
benzothiophene
acenaphtylene
<0,1
acenaphtene
<0,1
fluorene
<0,1
Phenanthrene
<0,1
anthracene
<0,1
fluoranthene
<0,1
pyrene
benzoaanthracene
chrysene
benzobfluoranthene
benzoepyrene
benzoapyrene
perylene
PCB153
PCB138
PCB180
Po-Lac
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
<0,05
-
-
-
-
-
-
-
-
-
-
<0,1
-
-
-
-
-
-
-
-
-
-
-
-
Organic
-
-
-
-
-
-
-
-
-
-
-
<0,1
-
-
-
-
-
<0,1
-
-
<0,1
-
-
-
-
-
<0,05
-
<0,1
<0,05
-
-
La Rochelle MARINA project
(1)
Study in semi-open environment (Six Sites & Three species)
•Dessalure = 1
•Treatment of fairing = 3
•Fuel (new) = 5
3
•Fuel (old) = 6
5
•Release of dredging = Semaphore BDM
•Control : Loix en Ré
Scallops, M. varia
6
1
BDM
3 species
Mussels, M.edulis
Oysters, C. gigas
 Quantification of contaminants &
Mesure of biological parameters (4)
 Determination of a harbour pertinent
specie to check the water quality
GST = oil pollution specific
La Rochelle MARINA project
(3)
NO SIGNAL !
*
Preliminary results  2020 …
Missing after the other results
Scallop aims towards the best specie
La Rochelle MARINA project
(4)
3
• Evaluate system water treatment
 System put in place since 2 years
• Analyses and waters sediments
• Dredging
To follow & to check the Quality
of Environment in harbour area
Water treatment pond of fairing,
La Rochelle Marina
60 years
Thank you for your attention
70 years
2000 years
Littoral Atlantic Coast & La Rochelle Marina Evolution
Thanks to:
Frédéric Muttin Hélène Thomas-Guyon Angélique Fontanaud Bertrand Moquay
Valérie Huet Armelle Combaud Thierry Guyot
Stéphane Le Floch Marianne Graber
Nicolas Lachaussée Philippe Pineau Philippe Rouger