actes du colloque sur les biotoxines marines proceedings

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CNEVA
ACTES DU COLLOQUE
SUR LES BIOTOXINES MARINES
PROCEEDINGS OF SYMPOSIUM
ON MARINE BIOTOXINS
J.MarcFREMY
Editor
Paris, 30 - 31 Janvier 1991
Centre National d'Etudes Vétérinaires et Alimentaires
SOMMAIRE
CONTENTS
Pages
^REFACE
1
MARINE BIOTOXINS : A SHORT OVERWIEW
} M. FREMY
3
PREMIÉRE PARTIE : ÉTUDES BIOCHIMIQUES ET TOXICOLOGIQUES
7
TOXIN VARIABILITY IN ALEXANDRIUM : MECHANISMS AND IMPLICATIONS
J M. ANDERSON
9
FATEOFPARALYTICSHELLFISHTOXINSDURINGACCUMULATIONANDDEPURATION
BYSHELLFISH
Y. OSHIMA
-
11
EFFECTS OF TEMPERATURE AND SALINITY ON CELLULAR SIZE AND THE
BIOCHEMICALPROFILE IN THE DINOFLAGELLATE ALEXANDRIUM LUSITANICUM
C CASAIS-LAINO andS GAFtCIA-MARTIN
13
TOXIC ALGAL BLOOMS DANISH COASTAL WATERS, A NEW PROJECT
H. RAVN {Ph D Project Leader)
19
BIOSYNTHESISOFDSPTOXINS. PRELIMINARY RESULTS.
M. NORTE, R. GONZÁLEZ and J. FERNANDEZ
21
PURIFICATION PAR F.L.P.C. DES PHYCOTOXINES PARALYSANTES GTX 2 /GTX 3
RESULTATS PRELIMINARES
C LAPEYRE.M. LEDOUX, M MORLAIX, C DELPONT, J.M. FREMY, P. LASSUS
29
DOMOIC ACID, A NEUROTOXIN PRODUCED BY THE PENNATE DIATOM
NITZSCHIA PUNGENS FORMA MULTISERiES : AN OVERVIEW OF SOME RECENT ECOLOGICAL
ANDPHYSIOLOGICALOBSERVATIONS
J. WORMS, 5. BATES, J.C SMtTH, P. CORMIER. C LEGER and K. PAULEY
35
FIELD AND EXPERIMENTAL STUDIES OF SHELLFISH CONTAMINATED BY
AN ALEXANDRIUM MINUTUM STRAIN.
M. LEDOUX, M. BARDOUIL, E- NEZAN, E. ERARD
43
LES TOXINES DE LA CIGUATERA
A M. LEGRAND
-
53
ICHTYOSARCOTOXISME DE TYPE CIGUATERA : ETUDE ÉCOTOXICOLOGIQUE ET MORPHOLOGIQUE
DEGAMS/FfiD/SCL/SrOX/CL/SAUXANTILLESFRANCAISES. (SAINT-BARTHÉLÉMY).
P BOURDEAUetM DURANDCLEMENT
61
ICHTYOSARCOTOXISME DE TYPE CIGUATERA : ETUDE DES POISSONS TOXIQUES
DES ANTILLES FRANCAISES : SAINT-BARTHELEMY ET SAINT-MARTIN
P. BOURDEAU
69
RECENT ADVANCES ON THE MODE OF ACTION OF MAITOTOXIN
M. DURAND-CLEMENT, G DIOGENEandS. PUISEUX-DAO
75
MODES OF ACTION OF THE TOXIC PRINCIPIE of GYRODINIUM CF. AUREOLUM
P. GENTIEN, G. ARZUL and F. TOULARASTEL
DEUXIEME PARTIE : ETUDES ANALYTIQUES
83
87
COMPARARON DES METHODES CHIMIQUES ET BIOLOGIQUES POUR LA DETERMINATION
DESTOXINES DIARRHEIQUES DANS LES MOULES
C MARCAILLOULE BAUT, P. MASSELIN and M. BOHEC
89
DOSAGE EN CL.H.P. DE L'ACIDE OKADAIQUE DANS DES EXTRAITS PHYTOPLANCTONIQUES
MA5SEUNP., MORLAIXM., BARDOUILM, LASSUS P. and L. LE DEAN
93
HPLC DETERMINATION OF DSP TOXINS
J.LSHEN, G GAN2LIN. andB. LUCKAS
101
EVALUATION OF YASUMOTO TEST FOR THE DETERMINARON OF DSP TOXIN IN SHELLFISH.
L TOTI, L CROO, D De MEDIO, 5. GIZZARELLI, M. Di PASQUALE, L OREFICE, A. STAZI
1 07
ADAM REAGENT FOR ACID LABELING. ASSESSMENT OF ADAM CONTENT IN AN UNKNOWN SOLUTION
L-G.M.Th. TUINSTRA,A.H. ROO5J.M.P VANTRIJP,M. KOCKEROLSand R. HARTEMINK
111
EXPERIMENTSWITH "IN SITU" SYNTHESISED ADAM
M. KOCKEROLS,J.MP VAN TRIJPand L.G.M Th TUINSTRA
118
MISES AU POINT RECENTES DE LA MÉTHODOLOGIE ANALYTIQUE DES PHYCOTOXINES MARINES :
LES TOXINES PARALYSANTES
A.D. CEMBELLA
119
HPLC DETERMINATION OF PSP TOXINS
G.THIELERT, I KAISER and B. LUCKAS
121
DETERMINATION OF PARALYTIC SHELLFISH POISONS BY PRE-CHROMATOGRAPHlC OXIDATION
AND HPLC
J.F. LAWRENCEandC MENARD
1 27
LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY FOR THE ANALYSIS OF MARINE TOXINS
M-A. QUILLIAMandS. PLEASANCE
131
AN ASSESSMENT OF THE SUITABILITY OF HPLC TECHNIQUES FOR MONITORING OF PSP AND DSP
ON THE EAST COAST OF ENGLAND
MJ WALDOCK, KM. EVANS, RJ LAWandT.W FILEMAN
137
ENZYME IMMUNOASSAYS FOR THE DETECTION OF SAXITOXIN
E. SCHNEIDER. E USLEBER, V. REN2. and G TERPLAN
145
IMPROVEMENT OF METHODOLOGY FOR PSP AND DSP DETERMINATION IN THE EEC
X. GOENAGA.X andPJ. WAGSTAFFE
151
MOYENS D'INVESTIGATION POUR CONFIRMER UNE INTOXICATION DE TYPE CIGUATERIQUE
j.P. VERNOUX
153
TROISIÉME PARTIE : PLANS DE SURVEILLANCE ET REGLEMENTATIOIM
163
REGULATION ON CONTROL MEASURES IN THE EEC
6. DAEL MAN and H. BELVEZE
165
WORLDWIDE REGULATIONS FOR MARINE PHYCOTOXINS
HP. VAN EGMOND, HJ. VAN DEN TOP
167
DIARRHEICSHELLFISH POISONS IN IRISH COASTAL WATERS : Programmeand problems 1984-1990
I. DOYLE, E. NIXONandD. JACKSON
173
OKADAIC ACID IN MUSSELS IN RELATION TO CURRENTS AND NUTRIENTS IN THE SEA
i. EDEBO, J. HAAMER, y. HU, 5 LANGE, X.P. U
175
MONITORING AND ANALYTICAL PROGRAMMES ON PHYCOTOXINS IN DENMARK
8. HALO, T. BJERGSKOV, H. EMSHOLM
181
REPHY : LE RESEAU FRANCAIS DE SUIVI DU PHYTOPLANCTON
C BELIN et J.P. BERTHOME
189
ECOTOXICOLOGICALSTUD1ESOFSOME RECENT A/.FX4A/DR/UMSP. OUTBREAKS IN FRANCE
f. NEZAN and G. PICLET
195
MARINE PHYCOTOXINS DETECTION IN ITALY
L VOLTERRA, P. M.8. GUCCÍ, M. BRUNO
203
SPATIAL DISTRIBUTION OF PSP TOXICITY IN THE MUSSEL RAFTS OF THE GALICIAN RÍAS (NW SPAIN) :
SOME MANAGEMENT STRATEGIES
A MARTÍNEZ, B. REGUERA, M.L. FERNÁNDEZ, A. MÍGUEZ, and E. CACHO
211
THE MONITORING OF HARMFUL ALGAL BLOOMS IN GALICIA (NW SPAIN)
6. REGUERA, M.J. CAMPOS, S FRAGA, J. MARINO, and I BRAVO
217
DSP CONTAMINATED SHELLFISH IN GALICIAN RÍAS ALONG 1990
A. GAGO. E. de la FUENTE, J.A. RODRÍGUEZ and A. IBAÑIEZ
225
DINOFLAGELLATE TOXICITY : HARMFUL EFFECTS, WARNING MEASURES AND RESEARCH TRENOS
IN PORTUGAL.
S. FRANCA
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.
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229