52 - Labtimes

page 52
Lab Times
2-2012
Products
Product survey: RNA extraction
Old and New Ways to RNA
RNA extraction is the first step in a multitude of analytical molecular biological techniques that require
high-quality RNA, free of RNAase, enzyme inhibitors, proteins and DNA.
U
ntil the middle of the last century,
RNA was isolated similar to DNA
by time-consuming and laborious
CsCl-centrifugation. Obviously too timeconsuming for K. S. Kirby who worked on
cancerogenic effects of tannin in the 1950s
at the Institute of Cancer Research in London. Inspired by biochemists using phenol
to extract proteins from crude extracts, Kirby tried applying a phenol-water mixture to
extract RNA. Kirby’s method has since been
slightly modified; however, the basic principles of phenol extraction, which is still quite
popular, remain the same.
The crude extract containing RNA and
other cellular components is treated with a
phenol-water mixture that adapts an acidic
pH of 4.8. Proteins are solved in the organic
phenol phase, while RNA molecules stay in
the aqueous phase and DNA accumulates in
the phenol-water interphase. Traces of phenol in the aqueous phase are subsequently
removed by one or two further chloroform
extraction steps. The upper water phase
containing the RNA is then removed from
the phenol phase with a pipette.
Since phenol and water have similar
densities, and phases may not clearly separate from each other or may even invert,
Kirby later refined his extraction method.
Instead of phenol, he used a phenol-chloroform mixture that gravitates to the bottom
of the reaction vessel due to chloroform’s
high density, with the water phase always
on top.
Single extraction step
Pietro Chomczynski and Nicoletta Sacchi further improved phenol-chloroform extraction in the late eighties by adding guanidinium thiocyanate (GTC) to the phenolchloroform mixture and lowering its pH to
4 with sodium acetate buffer. Chomczynski’s acid phenol method integrates all extraction steps into a single one, followed by
a simple alcohol precipitation of the extracted RNA. Due to its high yields, reproducibility, throughput and time saving, acid phenol extraction has become the gold standard for numerous home-made extraction
mixtures and individually-designed RNA
extraction protocols. Chomcynski was clev-
er enough to patent his GTC-Acidic phenol
solution, which is also found in commercial
RNA extraction reagents such as Trizol and
Tri as well as in some RNA extraction kits.
However, if you have ever extracted
RNA with acidic phenol, or even worse,
melted phenol, to produce a freshly prepared water-saturated phenol solution,
you will certainly agree that phenol is not
the most pleasant substance to work with.
The same holds true for chloroform and the
strange smelling isoamylalcohol. Hence,
RNA guys were happy when, in the 1990s,
small spin columns packed with silica gel
membranes became available and revolutionised RNA extraction.
Silica spin columns are, essentially,
small, solid-phase extraction columns containing silica as solid-phase material that
specifically binds RNA under defined salt
and buffer conditions. Extraction of RNA
with spin columns usually follows four simple, idiot-proof steps common to all solid
phase extractions: column-conditioning,
sample application, washing and elution.
To simplify things even more, the first step
may be omitted in many commercial kits
based on spin columns, starting directly
with the sample application.
Bead-based extraction
However, adsorbent-based RNA extraction methods are not restricted to silica spin
columns. More and more kit suppliers rely
on tiny beads made of RNA-adsorbent materials, such as polystyrene-latex, glass fibres, cellulose or silica, to extract RNA.
The basic steps of bead-based RNA isolation are as easy as extracting RNA with silica spin columns. The sample is first lysed in
buffer containing guanidinium thiocyanate
and exposed to the beads in small reaction
tubes. Subsequently, RNA-bound beads are
repeatedly washed and centrifuged to remove any contaminants. After washing, a
chaotropic salt-free buffer is added to release the RNA. Beads devoid of RNA are
then sedimented by a further centrifugation step and RNA is finally recovered from
the supernatant.
However, even the centrifugation steps
are dispensable if magnetic beads com-
posed of an iron oxide core, coated with
silica or other RNA-adsorbing materials, are
used. Instead of a centrifuge, a magnet that
may be embedded, for example, in a tube
stand, draws the beads to the bottom of the
reaction tubes during washing and elution.
Since all required washing and elution
steps of spin column and magnetic bead
extraction may easily be performed by the
nimble arms of a liquid handler, most automatic RNA extractors either work with
modified silica spin columns or bead-based
extraction kits. However, the time-consuming washing steps that lead to high consumptions of expensive RNA extractor tips
and wash solutions are still a major shortcoming of these techniques.
Immiscible filtration
A very interesting concept that avoids
repeated washing steps during RNA extraction with magnetic beads has recently been
presented by the group of David Beebe from
the University of Wisconsin (Berry et al.,
Lab Chip, 2011, 11, 1747). The group replaced all washing steps by a single traverse
of the bead-bound RNA through an immiscible fluid barrier in a process they call Immiscible Filtration Assisted by Surface Tension (IFAST).
This new technique may sound complicated in the first place, however, it is plain
simple. To apply IFAST, Berry et al. constructed an IFAST-plate consisting of three
wells (input-, immiscible phase- and output
well) connected in series by micro channels.
The input well is loaded with cell suspension, lysis buffer and magnetic beads, while
the output well contains the elution buffer.
Both wells are separated by the immiscible
phase well in the centre, filled with a special oil to prevent mixing of the liquids. During the washing step, a permanent magnet
draws the magnetic beads and the bound
RNA through the immiscible oil-phase into
the elution buffer of the output well. Since
hydrophilic contaminants cannot traverse
the oil phase, they are captured in the input
well. The whole IFAST-extraction procedure
takes only a few minutes, which is considerably faster than RNA extraction with conventional kits.
Harald Zähringer
2-2012
Products
Lab Times
page 53
Table 1: Devices and/or Kits for Automatic RNA Extraction
Company/Distributor Name of Device/Kit
Suitable samples / RNA type
Price [EUR]
5
High Throughput RNA purification kit in a
96 well format
2 plates / Sets of 96 samples of 10 to 5 × 10 cells
each
666.-
Absolutely RNA 96 Microprep kit, 10 plates
See above
10 plates Sets of 96 samples of 10 to 5 × 105 cells
each
2,780.-
Amsbio
ExpressArt QuickGene
FFPE tissue RNA isolation kit for the
Fujifilm QuickGene-Mini80 instrument
Isolation of total RNA from FFPE tissue samples
745.-
Analytik Jena
InnuPure C16
Nucleic acids are adsorbed onto magnetic
or paramagnetic particles
Total RNA from tissue samples, cells (eucaryotic and
procaryotic) and plant samples
14,900.-
Fully automated nucleic acid extraction based Total RNA from tissue samples, cells (eucaryotic and
procaryotic) and plant samples
on proven magnetic particle separation
35,000.-
Agilent Technologies
Absolutely RNA 96 Micro-
Extraction Technology
www.genomics.agilent.com prep kit, 2 plates
FFPE RNAready kit
Abingdon, UK
www.amsbio.com
Contact: [email protected]
Phone: +44 1235 828200
Life Science
Jena, Germany
InnuPure C96
www.bio.analytik-jena.de
Contact: lifescience@
innuPREP Tissue RNA
analytik-jena.de
Kit - IP
Phone +49 36 4177-9400
Beckman Coulter
Automated extraction of total RNA
Total RNA from a wide variety of tissue samples
138.670.2,670.-
innuPREP Plant RNA
Kit - IP
Optimised for automatically extracting
Total plant RNA
138.670.2,670.-
innuPREP Cell RNA Kit - IP
Pre-filter for fast, selective removal of DNA
(no DNase I digestion) greatly reduces
time required
Isolating of total RNA from eukaryotic and prokaryotic
cells (gram+ and gram– bacteria)
138.670.2,670.-
RNAdvance Cell v2
Extraction of total RNA with Solid Phase
Reversible Immobilisation (SPRI)
paramagnetic beads from 1-96 samples
in 96 plate format on Biomek FXp or
NXp liquid handlers
Cultured cells, total RNA
On request
Total RNA extraction with SPRI on Biomek
FXp or NXp span8 liquid handlers
Tissues, total RNA
On request
Formapure
RNA Extraction with Solid Phase
Reversible Immobilisation (SPRI)
paramagnetic bead-based technology
utilizing the Beckman Coulter Biomek
NXP or FXP Span-8 workstation
Formalin fixed, paraffin embedded sections;
total RNA or total nucleic acid
On request
RNAdvance Blood
RNA extraction with Solid Phase Reversible PAXgene stabilized blood samples, total RNA
Immobilisation (SPRI) paramagnetic beadbased technology on Beckman Coulter
Biomek FXp or NXp liquid handler
On request
geneMAG-RNA/DNA 96
geneMAG-RNA/DNA 480
Magnetic RNA/DNA purification kit compatible for KingFisher 96 or BioSprint 96
workstation
Isolation of total-RNA/DNA from 109 bacteria cells
185.725.-
AxyPrep MAG / FFPE /
DNA – RNA- miRNA Kit
Large: 384 preps
Medium: 96 preps
Magnetic beads-based technology
Maximum weight of 10 mg of any formalin-fixed,
paraffin embedded tissue sample
404.26
1,341.68
IMAG-12T Device
A hand held Magnetic Bead Separation
Device (MSD) for up to 12 tubes
Suitable for many molecular biology applications, where 691.88
automated platforms are not required
IMAG-96P Device
A hand held Magnetic Bead Separation
Device (MSD) for one 96 well microplate
(standard or deep well)
Suitable for many molecular biology applications, where 953.50
automated platforms are not in place
epMotion 5075 TMX
epMotion 5075 VAC
Small benchtop automated liquid
handling system /
Apply magnetic bead separation (TMX
version) or vacuum method (VAC) for RNA
purification methods
Total RNA, mRNA, viral RNA, using pretested Plug’n’Prep Please inquire
methods for various kit suppliers
illustra RNAspin 96 Kit
Simultaneous processing of 96 samples
via either centrifugation or vacuum-based
protocols
Cultured cells, tissue, bacteria or yeast
1,170.-
illustra RNAspin 96 Filter
Plate
Accessory product that facilitates the
use of tissues as input material for
96 well total RNA extraction with the
RNAspin 96 Kit
Processing of tissues with illustra RNAspin Mini 96
212.-
Krefeld, Germany
www.beckmancoulter.com
Contact: Christoph Krüll
Phone +49 2151 333 723
RNAdvance Tissue
[email protected]
Chemicell
www.chemicell.com
Contact: Cengiz Öztürk
Phone +49 30-2141481
[email protected]
Axygen Bioscience
Corning Life Sciences
Amsterdam, The
Netherlands
www.axygenbio.com
Contact: Joanne Woodward
Phone +44 7788 645292
[email protected]
Eppendorf
www.eppendorf.com
Contact: [email protected]
Phone +49-4053801-0
GE Healthcare
www.gelifesciences.com/
sampleprep
Contact: Sally Woods
Please contact your local
service department
page 54
Lab Times
2-2012
Products
Table 1: Devices and/or Kits for Automatic RNA Extraction
Company/Distributor Name of Device/Kit
Hamilton Bonaduz
Bonaduz, Switzerland
Contact: www.
hamiltoncompany.com
Extraction Technology
Suitable samples / RNA type
Price [EUR]
GenomicsStarlet
4 channel, manual (ML)
or autoload load (AL)
8 channel manual or
autoload
Total RNA extraction with 96 Nucleospin
kit from M&N
Total RNA from tissue and cells
(4 and 8 channel, manual or autoload load)
69,000.85,000.78,000.94,000.-
chemagicSTAR
Total RNA extraction out of PAXgene
Total RNA from blood in PAXgene tubes
blood tubes either 2 x 24 or 2 x 12 sample
per run with the chemistry from
Chemagen
149,900.-
MagMAX Express Magnetic Processing of magnetic magnetic beads
Particle Processor
Viral, blood, plant, tissue, cells
14,430.-
MagMAX Express-96 Deep
Well Magnetic Particle
Processor
Processing of magnetic beads
See above
39,270.-
iPrep Purification
Instrument
Processes up to 12 samples per run
plus a control. Integrated ChargeSwitch technology for inhibitor-free
purification
Viral samples, cultured cells, blood, and plant samples
28,930.-
Arrow / Automated RNA
Extraction
Magnetic beads
Total RNA from various samples /
Virus-RNA from sputum, stool, swabs, blood, tissue,
serum, plasma, cell pellets, urine
From 8,900.-
LabTurbo
Membrane columns
RNA from plant and human samples, virus, sputum,
stool, swabs, blood, tissue, serum, plasma,
cell pellets, urine
From 21,900.-
NucleoSpin 8 RNA
Silica-membrane technology
Tissue, cells /
Total RNA > 200 nt
On request
Silica-membrane technology
Tissue, cells /
Total RNA > 200 nt
On request
Magnetic-bead technology
Tissue, cells /
Total RNA > 200 nt
On request
NucleoSpin 8 RNA Blood
Silica-membrane technology
Whole blood /
Total RNA > 200 nt
On request
NucleoSpin 96 RNA Blood
Silica-membrane technology
Whole blood /
Total RNA > 200 nt
On request
MultiMACS M96 Separator The instrument works in combination with
a wide range of MultiMACS Kits
mRNA from cells, tissue samples, total RNA,
or whole blood
On request
MultiMACS M96thermo
Separator
See above / Heating functionality to heat
up samples on-column at 37°C or 42°C
mRNA from cells, tissue samples, total RNA,
or whole blood
On request
MultiMACS mRNA
Isolation Kit
Superparamagnetic Oligo(dT) MicroBeads
and MACS Columns
mRNA from cells, tissue samples, total RNA,
or whole blood (12 x 8 or 4 x 96 reactions)
On request
MultiMACS cDNA
Synthesis Kit
Superparamagnetic Oligo(dT) MicroBeads
mRNA from cells, tissue samples, total RNA,
or whole blood (12 x 8 or 4 x 96 reactions)
On request
PerkinElmer
chemagic Magnetic
Automated nucleic acid isolation;
Magnetic Bead based technology
Blood, stabilized blood, PAXgene blood,
serum/plasma, urine, stool suspension, swabs, cells,
tissue
On request
Contact: Christian Henze
Christian.henze@
perkinelmer.com
Contact: Jürgen Oster
Juergen.oster@
perkinelmer.com
chemagic Prepito
Compact benchtop system;
Magnetic bead based technology
Blood, serum/plasma, urine, stool suspension, swabs,
cells, tissue
On request
chemagic mRNA/gDNA Kit Magnetic bead based technology;
special
kit for chemagic MSM I
2 ml blood / m/RNA
On request
chemagic Total RNA Kits
Magnetic bead based technology;
kit for chemagic MSM I
2.5 ml PAXgene blood
On request
chemagic mRNA Direct Kit
Magnetic bead based technology;
kit for manual use with chemagic
Stands
Animal or plant tissue, cells /
Scalable for extraction of at least 100 μg mRNA/Kit
On request
chemagic DNA Blood Kits
special
Magnetic bead based technology;
several kits for chemagic MSM I
Blood / mRNA
On request
Life Technologies
www.lifetechnologies.com
Darmstadt, Germany
Contact: Anke Werse
debra.nickson@
lifetech.com
LTF Labortechnik
Wasserburg, Germany
www.labortechnik.com
Contact: Rudolf Walser
Phone +49 8382-98520
[email protected]
Macherey-Nagel
Dueren, Germany
NucleoSpin 96 RNA
www.mn-net.com
Contact: Michael Bußmann
Phone +49 2421969270 NucleoMag RNA
[email protected]
Miltenyi Biotec
Bergisch Gladbach,
Germany
www.miltenyibiotec.com
Contact:
[email protected]
Separation Module I
Chemagen Technologie
Baesweiler, Germany
www.chemagen.com
Contact: Andreas Künzel
Phone +49 2401-805500
andreas.kuenzel@
perkinelmer.com
2-2012
Products
Lab Times
page 55
Table 1: Devices and/or Kits for Automatic RNA Extraction
Company/Distributor Name of Device/Kit
Extraction Technology
Suitable samples / RNA type
Price [EUR]
Automation of silica-membrane based
extraction of nucleic acids from
1-12 samples at a time
Blood (human/animal), tissues, FFPE-tissues,
lipid tissues, microarray-tissues, cells, bacteria and
plant material
Please inquire
RNA purification based on silica
technology dedicated for use on the
QIAcube
Total RNA from cells and easy-to-lyse tissues
(e.g., kidney, liver, spleen)
Please inquire
BioRobot Universal
Medium- to high-throughput silicamembrane–based extraction of nucleic
acids in 96 well format
Blood, tissue, and cells using Qiagen kits
Please inquire
RNeasy 96 BioRobot
8000 Kit
Silica technology in a 96 well format
Total RNA from cells and easy-to-lyse tissues
(e.g., kidney, liver, spleen)
3,503.-
RNeasy 96 Universal
Tissue 8000 Kit
Silica technology + QIAzol in a 96 well
format
Total RNA from all types of tissues
4,513.-
QIAamp One-for- All
Nucleic Acid Kit
Silica technology in a 96 well format
Total RNA and genomic DNA from a variety of sample
types (whole blood, buccal swab, etc.)
3,935.-
QIAsymphony RNA Kit
Combines silica-based technology with
magnetic particles
Total RNA from animal cells, tissues, or FFPE tissues /
Total RNA including miRNA and other small RNAs after
proteinase K treatment
Please inquire
QIAsymphony PAXgene
Blood RNA Kit
Magnetic-particle technology
Total RNA, including miRNA, from blood
Please inquire
Roche Diagnostics
MagNA Pure 96 System
Mannheim, Germany
www.roche-appliedscience.com
Contact: Christian Knop
[email protected]
Magnetic glass particle technology
(silica-based purification)
Total RNA from whole blood, culture cells, fresh-frozen
tissue, RNAlater stabilized tissue, FFPE tissue, WBC
sediment of PAXgene tubes;
viral RNA from whole blood, serum, etc.
Please contact
local Roche
representative
MagNA Pure LC 2.0
System
Magnetic glass particle technology
(silica-based purification)
Total RNA from whole blood, blood cells, culture cells,
fresh-frozen tissue, FFPE tissue;
viral RNA from whole blood, serum, plasma
Please contact
local Roche
representative
MagNA Pure Compact
System
Magnetic glass particle technology
(silica-based purification)
Total RNA from whole blood, blood cells, culture cells,
fresh-frozen tissue, RNAlater stabilized tissue;
viral RNA from serum and plasma
Please contact
local Roche
representative
InviGenius
Fully automated system for DNA/RNA
extraction with magnetic bead based
EDTA- and citrate stabilized blood, serum, plasma,
urine, swabs, saliva, etc.
27,500.(German price)
Magnetic bead based, for use on the
InviGenius
Viral RNA from 200 μl blood, serum, plasma, rinse
liquid from swabs, cell-free body fluids
630.-
Magnetic bead based, for use on the
InviGenius
Viral RNA from 200 μl serum or plasma, cell-free body
605.fluids, rinse liquid from swabs, cell culture supernatants
and 50mg stool sample
InviMag Virus RNA Kit/
KFmL
(15, 75, 150 preps)
Magnetic bead based, for use on the
KingFisher mL (Thermo Scientific)
Viral RNA from up to 200 μl serum, plasma, cell-free
body fluids, etc.
90.324.598.-
InviMag Virus RNA Kit/
KF96
(1 x 96, 5 x 96 preps)
Magnetic bead based, for use on the
KingFisher 96/Flex (Thermo Scientific)
Viral RNA from up to 200 μl serum, plasma, cell-free
body fluids, etc.
237.50
828.-
InviMag Virus DNA/RNA
Mini Kit/KFmL
(15, 75, 150 preps)
Magnetic bead based, for use on the
KingFisher mL (Thermo Scientific)
Viral RNA (and DNA) from up to 200 μl serum, plasma,
cell-free body fluids, cell culture supernatants, etc.
73.50
294.50
529.50
InviMag Virus DNA/RNA
Mini Kit/KF96
(1 x 96, 5 96 preps)
Magnetic bead based, for use on the
KingFisher 96/Flex (Thermo Scientific)
Viral RNA (and DNA) from up to 200 μl serum, plasma,
cell-free body fluids, cell culture supernatant, etc.
261.1,300.50
Invisorb Virus RNA HTS
96 Kit/X
(4 x 96, 24 x 96 preps)
Membrane adsorption, filter plates for use
on X-tractor Gene, 96 well format
Viral RNA from up to 200 μl of plasma, serum, cell-free
body fluids etc.
774.4,567.-
Invisorb Virus RNA HTS 96
Kit/ep (2 x 96, 4 x 96,
24 x 96 preps)
Membrane adsorption, filter plates, for use Viral RNA from up to 200 μl of plasma, serum, cell-free
body fluids of human origin, etc.
on epMotion 5075 VAC (Eppendorf),
96 well format
415.735.4,337.-
InviMag Universal RNA
Mini Kit/KFmL
(15, 75 preps)
Magnetic bead based, for use on the KingFisher mL (Thermo Scientific)
Total RNA from up to 5 x 106 cells, up to 1.0 ml blood,
up to 20 mg tissue sample
110.400.-
InviMag Universal RNA
Mini Kit/KF96
(1 x 96, 5 x 96 preps)
Magnetic bead based, for use on the KingFisher 96/Flex (Thermo Scientific)
Total RNA from up to 5 x 106 cells, up to 1.5 ml blood,
10-40 mg tissue
310.1,122.-
Qiagen
QIAcube
Hilden, Germany
www.qiagen.com
Contact: Salim Essakali
RNeasy Mini QIAcube Kit
Phone +49 21032911343
[email protected]
Stratec Molecular
Berlin, Germany
www.invitek.de
Contact: Phone
InviMag Universal Kit/IG
+49 30 9489 2901/2910 (8 x 12 preps)
[email protected]
InviMag Virus RNA Mini
Kit/IG (8 x 12 preps)
page 56
Lab Times
2-2012
Products
Table 2: Kits for Manual RNA Extraction
Company/Distributor Name of Product
Active Motif
La Hulpe, Belgium
www.activemotif.com
Contact: Claire LaCroix
Phone +32 2 653 0001
[email protected]
5 PRIME
Hamburg, Germany
www.5PRIME.com
Contact:
Phone +49 40 3197927-0
[email protected]
Agilent Technologies
www.genomics.agilent.com
Amsbio
Extraction Technology
Suitable samples / RNA type
8
Price [EUR]
mTRAP Maxi mRNA
isolation kit
(6 and 5 x 6 reactions)
Complete kit based on a high affinity
Poly T gripNA probe (negatively charged
Peptide Nucleic Acids) and magnetic
beads
0,5-2 x 10 cells or 0.4-1.0 g tissue /
100-200 μg mRNA
On request
mTRAP Midi mRNA
isolation kit
(24 and 5 x 24 reactions)
--
0,5-1 x 107 cells or 50-200 mg tissue /
10-20 μg mRNA
On request
mTRAP Total mRNA
isolation kit
(12 and 5 x 12 reactions)
--
Up to 500 μg total RNA /
10-15 μg mRNA
On request
PerfectPure
RNA Blood Kit
Column-based silica membrane;
On-column DNase treatment
Whole blood and buffy coat
215.280.-
PerfectPure RNA Cultured
Cell Kit
Column-based silica membrane;
On-column DNase treatment
Cultured cells
63.- / 158.757.- / 700.-
PerfectPure RNA
RNA Tissue Kit
Column-based silica membrane;
On-column DNase treatment
Variety of tissue types
69.- / 174.- /
783.-
PerfectPure RNA
Fibrous Tissue Kit
Column-based silica membrane;
On-column DNase treatment
Fibrous tissue (heart, skeletal muscle or skin)
197.-
PerfectPure 96 Cell Kit
96-well format, silica membrane;
PerfectPure 96 Cell CS Kit centrifugation or vacuum processing
PerfectPure 96 Cell Vac Kit
5,000 to 5 million cells
1,240.1,240.1,220.-
Isol-RNA Lysis Reagent
Phenol/Guanidine-based Lysis Reagent
Variety of tissue types
149.-
Absolutely RNA
Nanoprep Kit
Isolate concentrated total RNA from the
smallest samples down to a single cell
Small samples of cultured cells (1–104 cells) or cells
harvested by laser capture microdissection (LCM)
315.-
Absolutely RNA
Microprep Kit
Purification of DNA-free total RNA from
cultured cells
Samples containing a very small number of cells
(1 cell to 5 x 105 cells)
245.-
Absolutely RNA
Miniprep Kit
Designed to produce high yield of pure
total RNA from cells or tissue samples
Purification of high-quality total RNA from small
samples of tissue or cultured cells
251.-
Absolutely mRNA
Purification Kit
Produces the highest yield of the purest
mRNA using a fast 20 minute protocol
Purification of mRNA from 100 μg total RNA
443.-
Absolutely miRNA Kit
Designed to produce high yields of pure
miRNA containing total RNA from cells or
tissue sample
Up to 25 mg tissue or up to 5 x 106 cells
243.-
SideStep Lysis and
Stabilization Buffer
Cell lysis and immediate stabilisation of
RNA and DNA for Q PCR analysis
10 ml buffer for 100 reactions from a variety of
mammalian cell lines
100.-
Side Step mRNA
Enrichment Kit
Exceptionally pure mRNA directly from
cells
A variety of mammalian cell lines
373.-
Side Step II Cell Lysis
Analysis Kit
Quantify RNA directly from cell lysates
From a variety of mammalian cell lines
205.-
Total RNA Isolation Mini Kit Spin column method
Mammalian cells and tissues including fibrous samples, 225.yeast and bacteria
Plant RNA Isolation
Spin column method
Plant samples from a wide variety of species and tissue
types
233.-
Spin column with on-column DNase
digestion
Isolation of total RNA from mammalian cell lines,
tissues and other samples
345.-
RNA isolation from FFPE tissue without
paraffin removal step
Isolation of total RNA from FFPE tissue samples
620.-
ExpressArt LCM RNAready
ExpressArt LBR RNAready
Spin column based extraction
Isolation of total RNA from laser microdissected samples 415.& cell lines (LCM); from tissue samples & bacteria (LBR)
Whole Blood RNA
Extraction Kit
Spin column based extraction
Isolation of total RNA from whole blood
230.-
Broad Range Total
RNA Isolation
Spin column based extraction
Isolation of total RNA from cultured cells and tissue
samples
175.-
Easy mRNA Kit for
RNA Isolation
Easy protocol based on several buffer
solutions
Isolation of mRNA from tissues and cells and also
from total RNA
300.-
MagSeq mRNA
Purification Kit
Separation with use of oligo dT magnetic
beads / Fast and easy protocol
Isolation of poly A+ RNA from total RNA
265.-
Total RNA Extraction Kit
Uses phenol and chloroform extraction
Isolation of total RNA from tissues and cells
240.-
Cartilage RNA Isolation Kit
Uses phenol and chloroform extraction
Isolation of total RNA from cartilage tissue and other
tissues
300.-
Mini Kit
Abingdon, UK
www.amsbio.com
ExpressArt RNAready Kit
Contact: [email protected]
Phone: +44 1235 828200 ExpressArt FFPE Clear
RNAready Kit
2-2012
Products
Lab Times
page 57
Table 2: Kits for Manual RNA Extraction
Company/Distributor Name of Product
Amsbio (continued)
Extraction Technology
Suitable samples / RNA type
Price [EUR]
MicroRNA Isolation Kit
Spin column based extraction
Isolation of microRNAs and other small RNAs from
cultured cells and tissues
175.-
Vantage Total RNA
Purification Kit
Spin column based extraction
Isolation of total RNA from animal cells, tissue samples, 211.blood, bacteria, yeast, fungi, plants and nasal or throat
swabs
Vantage microRNA
purification Kit
Spin column based extraction
Isolation of microRNA and small RNAs from cultured
cells, tissue samples, bacteria, plants and blood
205.-
EZ-RNA 96 Kit
High-throughput isolation in a 96-well
plate format that use filter plates
Isolation of total RNA from cultured cells
385.-
EZ-Blood RNA 96 Kit
High-throughput isolation in a 96-well
plate format that use filter plates
Isolation of total RNA from whole blood
440.-
RNA Bee
Reagent containing phenol and guanidinium thiocyanate
Isolation of total RNA from a large variety of biological
samples
180.-
RNA Stat-60
Reagent containing phenol and guanidinium thiocyanate
Isolation of total RNA, DNA and protein from tissues
and cells of human, animal, plant, yeast, bacterial and
viral origin
185.-
Ultraspec RNA
Reagent containing guanidinium salts
and urea
Isolation of total RNA from tissues, cells, bacteria,
plants and yeast
115.-
innuPREP RNA Mini Kit
Ready-to-use RNA isolated after only
15 - 40 minutes
Total RNA from a wide variety of different starting
materials
44.- / 175.- /
700.-
Universal, MIDI-format kit for extracting
total RNA
Total RNA
100.- / 270.- /
495.-
Based on Spin Filter technology
Total RNA and small RNA molecules, such as mRNA,
tRNA, rRNA, snRNA, miRNA, siRNA
44.- / 175.- /
700.-
innuPREP Blood RNA Kit
Selective removal of genomic DNA with no
DNase I digestion
Total cellular RNA from whole blood samples
44.- / 175.- /
700.-
innuPREP Blood RNA MIDI
Direct Kit
Based on the use of MIDI format spin filter
columns
Total cellular RNA from whole blood samples
100.- / 270.- /
495.-
innuPREP Plant RNA Kit
Selection of two integrated lysis buffer
systems
Total plant RNA
50.- / 200.- /
800.-
innuPREP Virus RNA Kit
Spin Filter columns with optimised binding
membranes
Viral ssRNA from serum, plasma or other cell-free
bodily fluids, etc.
38.- / 150.- /
600.-
Total RNA Maxi SI Isolation Silica-based spin columns / Lysis solution
containing chaotropic salts and phenol
Spin-Kit
Total RNA from bacterial culture, mammalian cell
culture, tissue and blood
202.70
Contact: see page 56
Analytik Jena
Life Science
Jena, Germany
innuPREP RNA MIDI
www.bio.analytik-jena.de
Direct Kit
Contact: lifescience@
innuPREP Micro RNA Kit
analytik-jena.de
Phone +49 36 4177-9400
AppliChem
Darmstadt, Germany
www.applichem.com
Contact:
[email protected]
Phone +49 6151-9357884
Bio & Sell
Total RNA Midi SI Isolation
Spin-Kit
Silica-based spin columns /
Lysis with chaotropic salts and phenol
Total RNA from, bacterial culture, cell culture, tissue,
blood
243.70
Total RNA Mini SI Isolation
Spin-Kit
Silica-based spin columns /
Lysis with chaotropic salts and phenol
Total RNA from bacterial culture, cell culture, tissue,
blood
88.60 /
296.95
Virus DNA/RNA Isolation
Spin-Kit
Silica-based spin columns /
Lysis with VirusAC-Solution and phenol
Virus RNA (or DNA) from 350 μl fresh or frozen blood
784.75
B&S RNA-Mini-Kit
Columns, no “shredder” necessary /
No DNAse digestion
Total RNA from tissue, from eukaryotic cells, from
bacteria
Starting at
0.99 / reaction
Guanidinium thiocyanate/Phenol
Tissue Monolayers / Suitable for adherent cells and
suspension cells
Starting at 69.- /
100 ml
Columns
Gram-negative and Gram-positive bacteria, tissue /
Adherent and suspension cells
Starting at 2.89
/ reaction
B&S RNA High efficiency
Liquid
Highly contaminated probes also for humin acid contamination probes
119.90 /
10 reactions
Norgen Total RNA
Spin column chromatography using silicon Mammalian cells and tissue, blood, plasma/serum,
bacteria, yeast, fungi, plants and more /
carbide resin (SiC)
All sizes of RNA including microRNA
230.- / 419.(50 / 100
reactions)
Spin column chromatography using silicon Mammalian cells and tissue, blood, plasma / serum,
bacteria, yeast, fungi, plants, & more / All sizes of RNA
carbide resin (SiC) / 96-well format for
automation / high throughput applications including microRNA
62.- (2 plates,
192 preps)
Feucht, Germany
www.bio-sell.de
B&S RNA tri-liquid
Isolation
Contact: [email protected]
Phone +49 9128 7243232 Micro-RNA Kit
BioCat
Purification Kit
Heidelberg, Germany
www.biocat.com
Norgen Total RNA
Contact: Elke Gamer
Purification 96-Well Kit
[email protected]
Phone +49-6221-7141516
Norgen FFPE Total RNA
Purification Kit for Formalin
Fixed Paraffin Embedded
Tissue
Deparaffinisation and Proteinase K
treatment followed by spin column
chromatography using silicon carbide
resin (SiC)
All types of formalin fixed paraffin embedded tissue /
All sizes of RNA including microRNA
304.- (50 rxns)
Norgen Preserved Blood
RNA Purification Kit I for
Tempus Blood RNA Tubes
or Kit II for PAX Blood RNA
Tubes
Lysis reaction followed by spin column
chromatography using silicon carbide
resin (SiC)
Blood preserved using PAXgene or Tempus Blood RNA
Tubes / All sizes of RNA including microRNA
390.- (50 preps)
page 58
Lab Times
2-2012
Products
Table 2: Kits for Manual RNA Extraction
Company/Distributor Name of Product
Extraction Technology
Suitable samples / RNA type
Price [EUR]
Norgen Urine Total RNA
Purification Maxi
Kit – slurry format
Spin column chromatography using silicon Urine samples
carbide resin (SiC)
All sizes of RNA including microRNA
450.- (50 x
5 ml preps)
Plasma/Serum Circulating
RNA Purification Kit
Spin column chromatography using silicon Serum or plasma prepared from blood collected on
carbide resin (SiC)
either EDTA or citrate
250.- /
12 x 5 ml preps
SeraMir Exosome RNA
Purification Kit
Exosome isolation followed by columnbased purification of exosomal RNA
Serum, plasma and tumor ascites fluid /
Free circulating RNA fragments including microRNAs
444.(20 reactions)
SeraMir TC Exosome RNA
Purification Kit
Exosome isolation followed by columnbased purification of exosomal RNA
Urine, spinal fluid, tissue culture media /
All sizes of RNA including microRNA
444.- (10 preps)
Norgen microRNA
Purification Kit
Spin column chromatography using silicon Cultured animal cells, small tissue samples and
carbide resin (SiC)
bacterial cells / Small RNA molecules (<200 nt)
188.(25 reactions)
geneMAG-RNA/DNA Kit
(15, 100, 500)
Isolation of total RNA/DNA with magnetic
silica particles
Blood, cells, bacteria, viruses
30.130.500.-
AxyPrep Multisource
RNA MiniPrep Kit,
4, 50, 250 preps/kit sizes
The mini spin/vac column allows samples
to be processed using either a centrifuge
or vacuum manifold
Animal tissue, plant tissue, cultured cells, bacteria,
yeasts, filamentous fungi
48.37
201.63
840.14
MidiPrep Kit,
2, 10, 25 preps/kit sizes
See above
See above
27.64 / 100.82
235.23
MaxiPrep Kit,
2, 10, 25 preps/kit sizes
See above
See above
36.85 / 145.89
464.88
AxyPrep Body Fluid Viral
DNA/RNA MiniPrep Kit,
4, 50, 250 preps/kit sizes
Viral nucleic acids are purified by binding
to a special AxyPrep column
Serum, plasma, ascites, cerebrospinal fluid, urine, cell
culture supernatants
23.74
98.95
445.27
AxyPrep Blood RNA
MiniPrep Kit,
4, 50, 250 preps/kit sizes
The mini spin/vac column allows samples
to be processed using either a centrifuge
or vacuum manifold
Anti-coagulated whole blood
61.04
299.67
1,220.94
AxyPrep MicroRNA
(miRNA) MiniPrep Kit,
4, 50, 250 preps/kit sizes
A purified cellular RNA subfraction, which
is highly enriched for lower molecular
weight species, such as miRNAs, siRNAs
and tRNAs is produced
Animal tissue, plant tissue, cultured cells
23.03
291.24
1,344.23
illustra RNAspin Mini Kit
20, 50, 250 preps
Simple and convenient format that is
suitable for all levels of expertise
Cultured cells, tissue, bacteria or yeast
108.15 / 222.967.-
illustra RNAspin Midi Kit
20 preps
High-quality total RNA due to the removal
of genomic DNA through on-column
DNase I treatment
Cultured cells, tissue, bacteria, and yeast
212.-
illustra QuickPrep Micro
mRNA Purification Kit
Utilise speed and selectivity of oligo(dT)cellulose spin column chromatography
to purify high-quality mRNA
mRNA from a wide variety of eukaryotic cells or tissue
samples, even from a single cell
523.-
Jena Bioscience
Total RNA Purification Kit
Isolation of total RNA by silica-gel
membrane adsorption
Total RNA from small amounts of various samples
including blood, animal and plant tissue, bacteria and
viruses
24.90.360.-
Life Technologies
PureLink RNA Mini
50 reactions
Silica-membrane column RNA purification
Total RNA from a wide range of cell and tissue types,
including animal, plant, and yeast cells, bacteria and
blood
223.-
Combines organic lysis capability of TRIzol
with silica spin-column format
See above
342.-
TRIzol Reagent 200ml
Ready-to-use, monophasic solution of
phenol and guanidine isothiocyanate
See above
409.05
Ambion Single Cell-to-CT,
50 reactions
Cells are simply lysed at room
temperature in ≤10 minutes;
an optional DNase digestion can be
performed concurrently during lysis
For samples containing between 1-10 cells
1,760.-
Ambion mirVana miRNA
Isolation Kit 40 reactions
Organic total RNA extraction followed by
purification on a glass fiber filter
Total RNA, including small RNAs, from virtually all
tissues and cells
302.- to 354.-
PureLink Viral RNA/DNA
Mini Kit
Spin column technology
Viral RNA from cell-free samples, such as serum,
plasma, and cerebrospinal fluid
180.-
BioCat (continued)
Contact: see page 57
Chemicell
Berlin, Germany
www.chemicell.com
Contact: Cengiz Öztürk
[email protected]
Phone +49-30-2141481
Axygen Bioscience
Corning Life Sciences
Amsterdam, Netherlands
www.axygenbio.com
Contact: Joanne Woodward
[email protected]
Phone +44 7788 645 292
GE Healthcare
Life Sciences
www.gelifesciences.com/
sampleprep
Contact: Sally Woods
Please contact your local
GE or distributor customer
service department
10, 50, 250 preps
Jena, Germany
www.jenabioscience.com
Contact: Bürk Schäfer
Phone +49 36416285000
[email protected]
Darmstadt, Germany
www.lifetechnologies.com
TRIzol Plus RNA PurificaContact: Anke Werse
[email protected] tion System 50 reactions
2-2012
Products
Lab Times
page 59
Table 2: Kits for Manual RNA Extraction
Company/Distributor Name of Product
Macherey-Nagel
Extraction Technology
Suitable samples / RNA type
Price [EUR]
Silica-membrane technology
Cells, tissue, bacteria, yeast / Total RNA > 200 nt
On request
Silica-membrane technology
Smallest amount of sample material /
Total RNA > 200 nt
On request
Silica-membrane technology
Cells, tissue, bacteria, yeast / Total RNA > 200 nt
On request
Silica-membrane technology
Cells, tissue, plants, reaction mixtures /
Small RNA (< 200 nt) and large RNA (> 200 nt) in
one or two separate fractions
On request
NucleoSpin miRNA Plasma Silica-membrane technology
Plasma, serum / RNA < 1000 nt
On request
NucleoSpin TriPrep
Silica-membrane technology
Cells, tissue, plants / Total RNA > 200 nt
On request
NucleoSpin RNA/Protein
Silica-membrane technology
Cells, tissue, plants / Total RNA > 200 nt
On request
NucleoSpin RNA Blood
Silica-membrane technology
Whole blood (fresh, frozen) / Total RNA > 200 nt
On request
NucleoSpin RNA Blood
Midi
Silica-membrane technology
Whole blood (fresh, frozen) / Total RNA > 200 nt
On request
NucleoSpin FFPE RNA
Silica-membrane technology
FFPE samples / Total RNA > 200 nt
On request
NucleoSpin FFPE
RNA/DNA
Silica-membrane technology
FFPE samples / Total RNA > 200 nt
On request
NucleoSpin RNA Plant
Silica-membrane technology
Plant material / Total RNA > 200 nt
On request
NucleoSpin RNA Clean-up
Silica-membrane technology
Phenol/chloroform extracts, reaction mixtures /
Total RNA > 200 nt
On request
NucleoSpin RNA
Clean-up XS
Silica-membrane technology
RNA containing solutions / Total RNA > 200 nt
On request
Straight A mRNA Isolation
Magnetight Oligo(dT) Particles, that
contain covalently attached oligo(dT)25 to
directly bind mRNA
The system effectively isolates poly(A)+ RNA from plant
and animal tissues as well as from cultured cells
467.-
See above
See above
607.-
μMAC mRNA Isolation Kit
Direct mRNA isolation from cells, tissue
samples, total RNA, or whole blood using
superparamagnetic Oligo(dT) MicroBeads
and MACS Columns
mRNA from cells, tissue samples, total RNA, or whole
blood
On request
AquaRNA
1ml, 30 ml
Column-free selective extraction with an
aqueous solution that inactivates RNases
Eukaryotic cells, animal tissue, microbial cells,
plant tissue, blood, small RNAs
16.215.-
peqGOLD RNAPure
Ready to use reagent for fast and easy
extraction of high-quality RNA
Total RNA from a wide variety of cells or tissue
See website
Ready to use reagent
Total RNA from fluidic samples, e.g. suspension cells,
blood, serum, plasma, biological fluids
See website
Reversible binding to PerfectBind silica
spin filters
Total RNA from fat-rich tissue
See website
peqGOLD MicroSpin Total
RNA Kit
Reversible binding to PerfectBind silica
spin filters
Total RNA from cells and tissues of human and vertebrate origin where starting material is limited
See website
peqGOLD Blood RNA Kit
Reversible binding to PerfectBind silica
spin filters
Total RNA extraction from fresh or anticoagulated,
e.g. heparin- or EDTA-treated, blood
See website
peqGOLD Plant RNA Kit
Reversible binding to PerfectBind silica
spin filters
Total RNA from plant cells and tissues, fungi and yeasts
See website
Total RNA from gram-positive and gram-negative
bacteria
See website
See website
NucleoSpin RNA II
Dueren, Germany
NucleoSpin RNA XS
www.mn-net.com
Contact: Michael Bußmann
Phone +49 2421969270 NucleoSpin RNA L
or 275
NucleoSpin miRNA
[email protected]
Merck Millipore
System
Darmstadt, Germany
www.merck4biosciences.com
Contact: GECustomer
Straight A’s mRNA [email protected] tion System plus Separation Stand
Miltenyi Biotec
Berg. Gladbach, Germany
www.miltenyibiotec.com
Contact: macs@
miltenyibiotec.de
MoBiTec
Goettingen, Germany
www.mobitec.com
Contact: Arne Schulz
[email protected]
Phone +49 551-707220
Peqlab Biotechnologie
Erlangen, Germany
www.peqlab.com
peqGOLD RNAPur FL
Contact: Emanuel Clausing
Phone +49 9131 6107020
peqGOLD HP Total RNA Kit
[email protected]
peqGOLD Bacterial RNA Kit Reversible binding to PerfectBind silica
spin filters
PromoCell
peqGOLD Viral RNA Kit
Reversible binding to PerfectBind silica
spin filters
Viral RNA from serum, plasma and biological fluids
RNA Isolation Kit I
Organic extraction and alcohol
precipitation
Total RNA from samples of human, animal, plant, yeast, 109.bacterial and viral origin
Organic extraction and alcohol
precipitation / BCP replaces volatile and
toxic chloroform
Total RNA from samples of human, animal, plant, yeast, 119.bacterial and viral origin
Heidelberg, Germany
www.promokine.info
RNA Isolation Kit II
Contact:
[email protected]
Phone +49 6221 64934-0
page 60
Lab Times
2-2012
Products
Table 2: Kits for Manual RNA Extraction
Company/Distributor Name of Product
Qiagen
Hilden, Germany
www.qiagen.com
Contact: Jörg Dennig
[email protected]
Phone +49 2103 2916326
EURx / Roboklon
www.eurx.com.pl
Gdańsk, Poland
Roboklon
Berlin, Germany
www.roboklon.com
Roche Diagnostics
Mannheim, Germany
www.roche-appliedscience.com
Contact: Christian Knop
[email protected]
Extraction Technology
Suitable samples / RNA type
Price [EUR]
RNeasy Kits (Micro, Mini,
Midi, Maxi, 96)
RNA purification based on silicamembrane technology
Total RNA from cells and easy-to-lyse tissue
(e.g., kidney, liver, spleen), yeast
Starting from
252.-
RNeasy Plus Kits (Micro,
Mini, 96)
Silica-membrane technology, including
gDNA Eliminator columns
Total RNA from cells and easy to lyse tissue
(e.g. kidney, liver, spleen), yeast
Starting from
286.-
Total RNA from all types of tissue
Starting from
174.-
RNeasy Plus Universal Kits Silica technology + QIAzol including gDNA
(Mini, Midi)
removal
RNeasy 96 Universal
Tissue Kit
Silica-membrane technology plus QIAzol in Total RNA from all types of tissue
a 96-well format
1,675.-
RNeasy Microarray Tissue
Mini Kit
Silica-membrane technology plus QIAzol
Total RNA from all types of tissue
334.-
RNeasy Protect Animal
Blood Kit
Silica-membrane technology
Total RNA from animal blood (stabilised)
406.-
RNeasy Protect Kits
Silica-membrane technology, including
RNA stabilisation
Cells, saliva, tissue, bacteria
Starting from
307.-
RNeasy FFPE Kit
Silica-membrane technology
Total RNA from formalin-fixed, paraffin-embedded
(FFPE) tissue sections
366.-
AllPrep Kits
Simultaneous purification of DNA, RNA, pro- Cells, tissue
tein based on silica-membrane technology
Starting from
423.-
miRNeasy Kits
(Micro, Mini, 96)
Silica-membrane technology plus QIAzol
Total RNA, including miRNA, from all types of tissue
and cells
Starting from
321.-
miRNeasy FFPE Kit
Silica-membrane technology plus QIAzol
Total RNA, including miRNA, from formalin-fixed,
paraffin-embedded (FFPE) tissue sections
371.-
QIAamp RNA Blood
Mini Kit
Silica-based spin columns
Total RNA from fresh whole blood; compatible with
common anti-coagulants
290.-
Universal RNA Purification
Kit (GeneMatrix) /
25, 100 preps
Spin column set
Human and animal tissues, cell cultures, plant tissue,
plant liquids, bacteria, yeasts, etc.
48.177.-
Universal RNA / miRNA
Purification Kit (GeneMatrix) / 25, 100 preps
Spin column set
Human and animal tissues (solid or liquid, but not
blood), cell cultures, plant tissue, plant liquids
53.197.-
DNA + RNA + Protein Extraction Kit (GeneMatrix) /
25, 100 preps
Spin column set
See above
62.229.-
Human Blood RNA Purification Kit (GeneMatrix) /
25 preps
Spin column set
Fresh human blood samples /
Heparin, citrate, EDTA conserved blood samples
53.-
High Pure RNA Isolation Kit Silica adsorption (spin column technology) Total RNA purification of blood, cultured cells, yeast,
and bacteria (scale: mini)
214.50
(Germany)
High Pure RNA Tissue Kit
Silica adsorption (spin column technology) Total RNA purification of tissue (scale: mini)
214.50
TriPure Isolation Reagent
Solution-based isolation
High Pure FFPE RNA
Micro Kit
Silica adsorption (spin column technology) Total RNA purification of 1-10 μm FFPE tissue sections
(scale: micro)
302.50
High Pure RNA Paraffin Kit
Silica adsorption (spin column technology) Total RNA purification of fresh-frozen or formalin-fixed
paraffin-embedded tissue (scale: mini)
439.-
High Pure miRNA
Isolation Kit
Silica adsorption (spin column technology) Total RNA and microRNA purification from animal tissue, 319.cell culture, formalin-fixed, paraffin-embedded tissue,
plant (scale: mini)
High Pure Viral RNA Kit
Silica adsorption (spin column technology) Viral RNA purification of serum, plasma, urine, other
body fluids, supernatant from cell culture (scale: mini)
mRNA Isolation Kit
Affinity purification
mRNA purification of cultured cells, tissues, total RNA
(scale: mini - midi)
263.-
mRNA Capture Kit
Affinity purification
mRNA purification of cultured cells, tissues, total RNA
(scale: micro - mini)
373.50
mRNA Isolation Kit for
Blood/Bone Marrow
Affinity purification
mRNA purification of whole blood/bone marrow
451.-
Quick Spin Columns for
Gel filtration
radiolabelled RNA purification, Sephadex G-25 /
Sephadex G-50
Up to 50 μl labelling mixture (Sephadex G-25)
Up to 100 μl labelling mixture (Sephadex G-50)
170.-
Mini Quick Spin
RNA Columns
20-75 μl labelling mixture
211.-
Gel filtration
Total RNA, DNA and protein purification of cultured cells, 121.50 (50 ml)
tissues, bacteria, yeast, blood, plant (mini, midi, maxi) 397.- (200 ml)
257.-
2-2012
Products
Lab Times
page 61
Table 2: Kits for Manual RNA Extraction
Company/Distributor Name of Product
Suitable samples / RNA type
Price [EUR]
InviTrap Spin Universal RNA Membrane adsorption
Mini Kit / 50, 250 preps
Total RNA from cells, tissue, FFPE tissue slices and
blood samples
202.- / 803.(Germany)
InviTrap Spin Cell RNA Mini See above
Kit / 50, 250 preps
Total RNA from mammalian cell cultures, Gram-positive
and Gram-negative bacteria cells and yeast cells
185.50
781.50
InviTrap Spin Tissue RNA
Mini Kit / 50, 250 preps
See above
Total RNA from up to 20 mg human or animal tissue
and paraffin embedded tissue samples
173.50
746.50
InviTrap Spin Blood RNA
Mini Kit / 50, 250 preps
See above
Total RNA from up to 1.5 ml of whole blood, 0.3 ml
buffy coat, up to 1 x 107 lymphocyte pellets
185.50
781.50
InviTrap Spin Plant RNA
Mini Kit / 50, 250 preps
See above
Total RNA from 100 mg plant material or 107 plant cells
Co-purification of viral RNA from phytopathogens
226.888.-
InviTrap RNA Cell HTS 96
Kit/C / 2 x 96, 4 x 96,
24 x 96 preps
Membrane adsorption, filter plates
Total RNA from 5 x 105 human and animal cells
449.788.50
4,604.-
InviTrap RNA Tissue HTS
96/C / 2 x 96
Membrane adsorption, filter plates
Total RNA from 1 - 10 mg tissue sample
468.- / 858.4,640.-
InviTrap RNA Plant
HTS 96 Kit/C /
4 x 96, 24 x 96 preps
Membrane adsorption, filter plates
Total RNA from up to 50 mg of plant material
708.50
4,088.-
Invisorb Spin Virus RNA
Mini Kit / 50, 250 preps
Membrane adsorption in capped spin
columns
Viral RNA from plasma, serum, blood, tissue,
stool samples, mammalian cells, cell culture
supernatants, rinse liquid from swabs
152.658.-
RNAsure Virus System (RNA- Purification with spin columns
sure Spin Virus Kit + Protection Module I) / 50 preps
Viral RNA from 200 μl of virus – containing samples of
serum and plasma or 100 μl blood samples
375.275.-
RTP DNA/RNA Virus Mini
Kit / 50, 250 preps
Pre-filled extraction tubes /
Purification with spin columns
Viral RNA (and DNA) from up to 200 μl of serum, plasma, 164.other cell-free body fluids, cell culture supernatants, etc. 689.-
Invisorb Virus RNA
HTS 96 Kit/C
Membrane adsorption, filter plates
Viral RNA from up to 200 μl of plasma, serum,
cell-free body fluids of human origin, etc.
415.- / 735.4,337.-
Invisorb Virus RNA HTS
96 Kit/V / 2 x 96, 4 x 96,
24 x 96 preps
Membrane adsorption, filter plates;
96-well format, using a vacuum manifold
Viral RNA from up to 200 μl of plasma, serum,
cell-free body fluids of human origin, etc.
400.716.4,254.-
PowerPlant RNA
Isolation Kit
Bead beating technology combined with
silica spin filter tubes
Most difficult plant types, including strawberry leaf,
cotton leaf, cotton seeds and pine needles
4.95 /prep
RNA PowerSoil Total RNA
Isolation
Inhibitor Removal Technology (IRT) for isolating total RNA from up to 2 grams of soil
Total RNA from all common soil types
11.- /prep
PowerBiofilm RNA
Isolation Kit
Chemical and mechanical lysis; Inhibitor
Removal Technology (IRT) to remove the
concentrated inhibitors
Nucleic acids from biofilm samples
8.50.- /prep
PowerWater RNA
Isolation Kit
Bead tubes for optimal lysis and Inhibitor
Removal Technology (IRT)
RNA from filtered water samples, including turbid waters 7.90 /prep
Hi Yield RNA Pure Kit
RNA Pure Buffer ensures easy binding of Up to 100 μl of RNA products
DNA to the glass fiber matrix of spin column
1.42 /prep
CellAmp Whole Transcriptome Amplification Kit
(Real Time) / 50 preps
Direct RNA prep and cDNA synthesis to
Mammalian cells or total RNA
prepare template from few cells (2-200) or
limited RNA amount (< 20 ng)
375.-
CellAmp Direct RNA Prep
Kit for RT-PCR (Real Time)
/ 200 preps
Direct preparation of RNA template to be
analysed by RT-qPCR (one or two step)
96-well cell culture should be used as sample to
prepare RNA lysate
180.-
CellAmp Direct Prep Kit for Direct preparation of RNA template to be
RT-PCR (Real Time) & Pro- analysed by RT-qPCR and proteins for
tein Analysis / 200 preps western blot analysis
96-well cell culture should be used as sample to prepare RNA and proteins lysate
190.-
Thermo Fisher
Scientific
GeneJET RNA Purification
Kit / 50, 250 preps
Total RNA from mammalian cell cultures and tissues,
whole blood, bacteria, yeast, insects
175.- / 750.(Germany)
St. Leon-Rot, Germany
www.fermentas.com
Contact: Luiz Correa
Techservice.emea.
genomics@thermofisher.
com
Phone +49 6227-356790
GeneJET Whole Blood RNA Silica-based membrane spin column
Purification Mini Kit /
50 preps
Total RNA from whole blood and related body fluids,
bone marrow, buffy coat
200.(Germany)
GeneJET Plant RNA Purifica- Silica-based membrane spin column
tion Mini Kit/50, 250 preps
Total RNA as well as mitochondrial, chloroplastidial, viral 200.- / 800.(Germany)
and bacterial RNA from a wide variety of plant species
GeneJET Viral DNA and
RNA Purification Kit /
50 preps
Viral nucleic acids from various human and animal
liquids
165.(Germany)
Viral nucleic acids from human plasma or serum samples for in vitro diagnostic purposes
200.(Germany)
Stratec Molecular
Berlin, Germany
www.invitek.de
Contact:
[email protected]
Phone +49 30 9489 2901
or 2910
Süd-Laborbedarf
Gauting, Germany
www.suedlabor.de
Contact: Uli Schaffar
Phone: +49 898506527
[email protected]
Takara/Clontech
Takara Bio Europe
St-Germain-en Laye, France
www.takara-bio.eu
Contact: Francois-X. Sicot
[email protected]
Phone +33 1 3904 6880
(Europe)
Extraction Technology
Silica-based membrane technology in the
form of a convenient spin column
Silica-based membrane spin column
Viral DNA/RNA Purification Silica-based membrane spin column
Kit / 50 preps