curriculum vitae - Qatar University

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CURRICULUM VITAE
Dec. 2012
1-PERSONAL PROFILE
First name and surname: Samir Mohamed JAOUA
Birthday : 28/08/1959 Sfax [Tunisia], married and father of 3 children
Address: Qatar University, College of Arts & Sciences. Department of Biol. & Environ. Sc. P.O.Box 2713.
Doha- Qatar
Office : B 207 Tel: 974 44034536, Fax: 97444034531, Mobile: 974-66406828.
E-mail addresses: [email protected]; [email protected]
2-PROFESSIONAL/ACADEMIC EXPERIENCES
-Since September 2012: Professor and Head of the Department of Biol. & Environ. Sc. at Qatar
University - College of Arts & Sciences. Doha Qatar
-Since September 2011: Professor and Program Coordinator of the Master Sc. in
Environmental. Sc. at Qatar University - College of Arts & Sciences. Doha Qatar
-Since September 2009: Professor at Qatar University - College of Arts & Sciences. Department
of Biol. & Environ. Sc., Doha Qatar
-Since May 2009: Qualification of Professor of France Universities “Section 64: Molecular
Biology and Biochemistry”, France
-December 1996 - August 2009 : Professor, University of Sfax- Tunisia
- Teaching mainly M. Sc. courses: M. Sc. in Biol. Eng., M. Sc. in Mol. & Cell Biol., M. Sc. in Environ.
Biotechnol., M. Sc. in Aquatic Biodiv. and Resources., M. Sc. in Biotechnol. & Immunol.
- Director of the Laboratory of Biopesticides (2001-2009)
-1992-1996: Associate Professor: University of Sfax- Tunisia
- Teaching Molecular Biology, Molecular Genetics and Genetic Engineering for B.Sc. and
Engineering
- Director of the Research Unit of Metabolites Production (1993-1996)
-Jan - Oct 1995: Invited in the Lab. Genetics of Pr Mark Von Montagu, Univ. of Gent Belgium
-Jan - Mar 1989: Post-Doc in GBF, Braunschweig- Germany
-Apr 1989 - Dec 1991: Post-Doc in Ciba Geigy Basle, Switzerland
-Jan 1987 - Dec 1988: Assistant-Professor at the Faculty of Sciences & Techniques, Rouen, France
-Oct 1986: Ph. D Defense at the University of Technology of Compiegne, France
3-ACADEMIC QUALIFICATIONS :
-Bachelor in General Biology
June 1982, at the Faculty of Sciences and Techniques. Sfax. Tunisia
-ENGINEER, in Biology Engineering
July 1984, at the Faculty of Sciences and Techniques. Sfax. Tunisia/University of Technology of
Compiegne, FRANCE
-MASTER DEGREE, in Microbiology, Enzyme Engineering & Bioconversion
June 1984, at the University of Technology of Compiegne, FRANCE
Microbiology, Molecular genetics
-Ph. D in Microbiology, Enzyme Engineering & Bioconversion
Speciality: Molecular Genetics
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October 17th 1986, at University of Technology of Compiegne, FRANCE
Distinction: Very Honourable
Microbiology, Molecular genetics
-DOCTOR ES-SCIENCES
May 11th 1991, at the Faculty of Sciences of Tunis, Tunisia
Distinction: Very Honourable
Biotechnology, Molecular genetics
100 % of the research activities carried out in: France (2 years), Switzerland (2.5 years) and
Germany (3 months) .
4- SKILLS
-25 years of experience in the fields of Molecular Biotechnology and Genetic Engineering in different
countries: France, Switzerland Belgium, Germany, Tunisia and Qatar
5- COMMITTEE PARTICIPATION mainly during the 3 last years
-2011-2012:
-Chair of the Qatar University Academic Promotion Committee
-Member of CAS graduate committee
-Member of CAS PLO assessment committee
-Member of the External Relation committee (Dept. Biol & Env .Sc., CAS, QU, Qatar)
-Member of the recruiting committee (Dept Biol And Env Sc., CAS, QU, Qatar)
- Member of the academic committee (Dept Biol And Env Sc., CAS, QU, Qatar)
-2010-2011:
-Member of QU Academic Promotion Committee
-Chair of External Relation committee of the Depart. Biol. and Environ. Sc (CAS)
-Chair of the recruitment committee of Professors of the Tunisian Ministry of Higher Education, Scientific
research and Technology
-2009-2010:
-Member of CAS Academic Promotion Committee and strategic plan committee
-Chair of Research committee of the Depart. Biol. and Environ. and sub- committees co-chair
-Qatar University, CAS Strategic plan committee
-Co-Chair of Valorization and Biotechnology transfer sub-committee (External relation committee) of the
Depart. Biol. and Environ. Sc (CAS)
- Co-Chair of Outreach sub-committee (External relation committee) of the Depart. Biol. and Environ. Sc
(CAS)
-Member of Department Accreditation sub-committee of the Depart. Biol. and Environ. Sc (CAS)
-2008-2009: Member of the recruitment committee of Assistants and Assistant professors of the
Tunisian Ministry of Higher Education, Scientific research and Technology
-2004-2009: Member of the recruitment committee of researchers of Pasteur Institute of Tunis
-1996- 2009: Member of PhD, Habilitation and Master degree committees of the National School of
engineers, Sfax, Tunisia
- 1996- 2009: Member of PhD committees of the National School of engineers, Sfax, Tunisia
- 2000-2009: Member of several Master degree committees in Tunisia
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6- COMMUNITY SERVICE AT QATAR UNIVERSITY [2009-2011]
-2009-2011: Academic advisor of 6 students of the Environ. Sc Major, Qatar Univ CAS
-2010-2011: Production of High-value recombinant Enzyme Taq DNA polymerase by genetic Engineering,
replacing the very expensive one, used successfully in the Department. Biol. & Environ. Sc. Doha Qatar
-2009-2011: Seminars and workshop for the department
-2010-2011: Member of inspectors’ team for Biosafety, Ministry of Environment, Qatar
-2010: Member of the committee of Environmental sustainability, Ministry of Environment, Qatar
-2010: Participate to the continuous upgrading secondary schools teachers by giving lectures and
practical/labs of Genetic Engineering and Microbiology to secondary school teachers
-2010: Giving lectures of Genetic Engineering to secondary school students in their schools in Doha
-2010: Supervision of research projects of 3 young secondary school students, from Doha French School
7-MEMBERSHIP
- April 2011-2013: International collaborative researcher of the university of Tsukuba- Japan
-Since January 2005: North Africa, then Tunisia correspondent for the AUF (Agence Universitaire de la
Francophonie –BioVeg, Belgium), Biotechnology
-Editorial board member of Journal of Biopesticides
-Member of the Tunisian Society of Microbiology STM
-Member of COST862: Consortium of science and Technology (European Science Foundation)
-Member of the Tunisian National Gene Bank
-Reviewer of many international scientific journals: J. Appl. Microbiol., Lett. Appl. Microbiol., FEMS
Microbiol. Lett., J. Inv. Pathol., Mol. Biotechnol. , .....
8-TEACHING ACTIVITIES
1. Master Sc. in Environmental Sc. BIOL505, CAS Qatar Univ. Since 2011-2012
2. Bachelor in Environ. Sc.: Course of Molecular Analytical Techniques- BIOL452, CAS Qatar Univ. Spring
2012
3. Bachelor in Biological Sc.: Course of Genetic Eng. BIOL412, CAS Qatar Univ. Since 2009
4. Bachelor in Environmental Sciences: Course of Biotechnology and Bioremediation BIOL443, CAS
Qatar Univ. 2011
5. Bachelor in Environmental Sciences: Course of Microbiology BIOL241, CAS Qatar Univ.Since 2011
6. Bachelor in Biology: Course of Biotechnology, BIOL442, CAS Qatar Univ. 2010
7. Bachelor in Environ. Sciences: Course of Molecular and Cell Biology, BIOL310, CAS Qatar Univ.
Fall2010
8. Master Sc. in Environmental Biotechnology, Higher Inst. of Biotechnol. Sfax, Tunisia:
Course of Molecular Genetics: 2007-2009
9. Master Sc. in Molecular and Cell Biology of the Fac. Sc. Sfax Tunisia:
Course of Molecular Biology: 2000-2009
10.
Master Sc. in Aquatic Biodiversity and resources of the Fac. Sc. Sfax Tunisia:
Course of Molecular Genetics: 2004-2009
11.
Master Sc. in Biology Engineering of the National college of Engineers of Sfax:
Course of Applied Microbiology: 1995-2009
12.
Master Sc. in Biology Engineering of the National college of Engineers of Sfax:
Course of Molecular Biology: 1995-2009
13.
Master Sc. of Biotechnology and Immunol. Appli. to transmissible diseases of the Fac. of
Pharmacy of Monastir, Tunisia: Course of Molecular Biology: Since 2000
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14.
Master Sc. in Biology of the Faculty of Sc., Univ. Saint Joseph Beirut Lebanon:
Course Microbiological Engineering: 2001-2007
15.
Bachelor of Life and earth of the Fac. Sc. of Sfax, Tunisia:
Course of Genetic Eng.: 2003-2007
16.
Bachelor of Natural Sciences of the Fac. Sc. of Sfax, Tunisia:
Course of Molecular : 1992 - 2000 : Genetics
17.
Biology Engineering of the National college of Engineers of Sfax: Course of molecular Biology:
1992
18.
Master Sc. of Molecular Biology of the Fac. Sc. Tunis, Tunisia:
Course of Molecular Biology: 1993
19.
Master Sc. of Biology Engineering of the National college of Engineers of Sfax:
Course of Applied Microbiology: 1995-2009
20.
Biology Engineering of the National college of Engineers of Sfax:
Course of Genetic engineering: 1997-1999
21.
Course of Microbiology, Faculty of Sciences, Rouen France: 1987-1988
9-PH. D DOCTORATES AND HABILITATIONS DEFENDED UNDER THE SUPERVISION
OF S. JAOUA:
Samir Jaoua has been the supervisor of the following 11 PhD students [+PhD defense dates]:
-Dr Slim Tounsi, PhD, 2004
-Dr Slim Tounsi , Habilitation
-Dr Ameur Cherif, PhD, 2005
-Dr Aida Hmida, Ph. D, 2006
-Dr Lobna Abdelkefi, PhD, 2007
-Dr Raida Zribi, PhD, 2008
-Dr Souad Rouis, Habilitation, 2008
-Dr Fakher Kamoun, PhD, 2009
-Dr Imen saadaoui, PhD, 2010
-Dr Mariam Dammak, PhD, 2010
-Dr Olfa Kilani Feki, PhD, 2011
-Dr Anis Ben Hsouna, Ph. D, 2011
-Dr Sameh Sellami, Ph. D, 2011
-Other 2 PhD will be defended in 2012 and 1 PhD in 2013
10- RESEARCH ACTIVITIES :
General Research topics:
Molecular and Environmental Biotechnology
Microbial Genomics and Proteomics and applied Microbiology
 Gene bank and Culture collection of Microbial strains producing bioactive molecules “BIO”
[Bioinsecticides, Biofungicides, Antibiotics, antioxidants...] or degrading compounds
[hydrocarbon]
 Gene cloning, manipulation and homologous & heterologous expression
 Production of metabolites by Fermentation at different scales
Study of the structure/ function of Proteins
Years 1987 and 1988 :
Assistant-Professor at the Faculty of Sciences and Techniques. Rouen. France.
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-Teaching Molecular Biology and Microbiology
-Research Subject : Gene transfer systems to Myxococcus xanthus
Laboratory of Microbiology. URA 203 CNRS. BP 118. 76821 Mont-Saint-Aignan cedex France
Years : 1989, 1990 and 1991 :
Post-Doc Researcher at Ciba-Geigy L.t.d (Novartis) Basle. Switzerland.
Department of Biotechnology, Pharmaceutical Research Division, K681-3-08, CH4002, Basle,
SWITZERLAND.
Subject : Development of a genetic system of DNA transfer to Sorangium cellulosum, a myxobacterium
producing a fongicide, Soraphen. Genes investigation. Antibiotic production improvement by genetic
manipulation.
January – October 1995 :
Visitor in the Laboratory of Molecular Genetics of Pr Marc Van Montagu. University of Gent. K.
Ledeganckstraate 35. 9000 Gent. Belgium. Supported by a Post-Doctoral fellow from the European
Community.
Research subject : MOLECULAR STUDY OF THE OSMO-REGULATION IN POTATO PLANTS: Isolation,
characterization, and chromosomal location of a gene encoding the delta1-pyrroline-5-carboxylate
synthetase, in Arabidopsis thaliana. Molecular study of the osmo-regulation.
From January 1992 to August 2009:
Associate-Professor (1992-1996) Research activities at the Centre of Biotechnology of Sfax, Tunisia.
Professor (Since December 23rd, 1996) Research activities at the Centre of Biotechnology of Sfax,
Tunisia.
From September 2009:
-Professor at the College of Arts & Sciences. Department Biol. & Environ. Sc. Doha Qatar
-Program coordinator of the Master of Science in Environmental Sciences
-Skills development:
In the department, we first of all tried to improve and update the basic skills of the technicians
interested in our research topics. At least 2 technicians are now able to carry out research
independently..
-Research programs:
As a team, we started a research program in Microbial and Environmental Biotechnology:
-Production of Bioactive Molecules and compounds not polluting for the environment: antibiotics,
fungicides, bioinsecticides, ...
-Bio-Degrade Polluting Molecules and compounds.
In the frame of Qatar vision 2030 for a sustainable Qatari Environment, our action plan consisted in
establishing and setting up a microbial gene bank. In the first stage, a culture collection of more than 500
strains of Bacillus thuringiensis, a friendly biopesticide producing bacterium, has been set up. These
biopesticides will be developed towards a sustainable Qatari and Gulf Environment free from chemical
pesticides. So, this collection is being used for several projects but will be useful in term of research
opportunities Master, PhD, postdoc,… but also industrial opportunities ..
We have started in parallel another project dealing with the isolation of hydrocarbon degrading
bacteria… We have now a strain that can grow and degrade 70% diesel…
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Another NPRP project will start on December 2011 : Mycosafe: Control of food-borne mycotoxins in
Qatar
Research grants: Since 2010
-QU Internal grant QUUG-CAS-DBS-09/10-7: Apr 2010- March 2012
PI: Prof Samir JAOUA
-QNRF-UREP 08 - 077 - 1 - 012: 2011: PI: Prof Samir JAOUA
-QNRF- UREP 09 - 019 - 1 : 2011-2012: PI: Prof Samir JAOUA
-Student Grant- QU- OAR- QUST-CAS-BES-10/11-3 – 2011 : PI: Prof Samir JAOUA
-Contributing in an NPRP 27-6-7-24 : Ended 2011
-NPRP No.: 09 - 705 - 4 - 025 - Co-PI: Samir JAOUA -- Dec2010 - Nov2013
-NPRP No.: 04 - 259 - 2 - 083 ……………- LPI: Samir JAOUA -- Dec 2011-Nov2015
11-University and community service at Qatar University (2009-2012)::
-1- Internal department seminars
-Many seminars done for the department week- seminars
-Many Seminars for the graduate project students
- Qatar Career Fair 2011---11-April 2011: Presentation on behalf of the Head of the Dept. of Biol. & env
Sc, of the programs of the department
-2-Organization of the 1st Workshop for Lab Technicians on January, 6th 2011
Title: THE BASIC SKILLS OF THE BIOLOGY WORKPLACE: Towards the research lab accreditation
-3-A New Research Lab of Biotechnology is set up
-4-New fermentor [New-Brunswick] for the first time at CAS- Qatar university –May 2011
-5- Home- made recombinant Taq DNA polymerase: [Biotechnology and Genetic engineering], with
outstanding benefits for the department:
We produce each semester more than 15,000 Units of Taq DNA polymerase enzyme for PCR and DNA
amplifications techniques . This is considered as an important Biotechnological application.
12-INTERNATIONAL PUBLICATIONS in Peer-reviewed Journals with Impact
Factors > 1:
1*BRETON A M, JAOUA S and GUESPIN-MICHEL J F (1985)
Transfer of plasmid RP4 to Myxococcus xanthus and evidence for its integration into the chromosome
J. Bacteriol. 161, 523-528
2*JAOUA S, BRETON A M, YOUNES G and GUESPIN-MICHEL J F (1986)
Structural instability and stabilization of IncP-1 Plasmids integrated into the chromosome of Myxococcus xanthus
J. Biotechnol. 4, 313-323
3*JAOUA S, GUESPIN-MICHEL J F and BRETON A M (1987)
Mode of insertion of the broad host range plasmid RP4 and some of its derivatives into the chromosome of
Myxococcus xanthus
Plasmid 18, 111-119
4*SAULNIER P, HANQUIER J, JAOUA S, REICHENBACH H and GUESPIN-MICHEL J F (1988)
Utilization of IncP1 plasmids as vectors for transposon mutagenesis in Myxobacteria.
J. Gen Microbiol. 134, 2889-2895
5*JAOUA S, LETOUVET-PAWLACK B, MONNIER C and GUESPIN-MICHEL J F (1990)
Mechanism of integration of the broad host range plasmid RP4 into the chromosome of Myxococcus xanthus
Plasmid 23, 183-193
6*JAOUA S, NEFF S and SCHUPP T (1992)
Transfer of mobilizable plasmids to Sorangium cellulosum and evidence for their integration into the chromosome
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Plasmid 28, 157-165
7*SAVOURE A, JAOUA S, HUA X J, ARDILES W, VAN MONTAGU M and VERBRUGGEN N (1995)
Isolation, characterization, and chromosomal location of a gene encoding the delta1-pyrroline-5-carboxylate
synthetase, in Arabidopsis thaliana.
FEBS Lett. 372, 13-19
8*JAOUA S, ZOUARI N, TOUNSI S and ELLOUZ R (1996)
Study of particular delta-endotoxins produced by three isolated strains of Bacillus thuringiensis
FEMS Microbiol. Lett. 145, 349-354
9*ZOUARI N and JAOUA S (1997)
Purification and immunological characterization of particular delta-endotoxins from three isolated strains of
Bacillus thuringiensis
Biotechnol. Lett. 19, 825-829
10*ZOUARI N, DHOUIB A, ELLOUZ R and JAOUA S (1998)
Nutritional requirements of a Bacillus thuringiensis subsp. Kurstaki strain and use of Gruel hydrolysate for the
formulation of a new medium for delta-endotoxin production
Appl. Biochem. Biotechnol. 69, 41-52
11*ZOUARI N and JAOUA S (1999)
Production and characterization of metalloproteases synthesized concomitantly with delta-endotoxin by Bacillus
thuringiensis subsp. Kurstaki strain grown on gruel-based media
Enz. Microb. Technol. 25, 364-371
12*ZOUARI N and JAOUA S (1999)
The effect of complex carbon and nitrogen, salt, Tween-80 and acetate on delta-endotoxin production by a Bacillus
thuringiensis subsp. Kurstaki
J. Ind. Microb. Biotechnol. 23, 497-502
13*TOUNSI S, J’MAL A, ZOUARI N and JAOUA S (1999)
Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis
Biotechnol. Lett. 21, 771-775
14*CHERIF A, OUZARI H, DAFFONCHIO D, CHERIF H, BEN SLAMA K, HASSEN A, JAOUA S and BOUDABBOUS A (2001)
Thuricin 7 : A novel bactriocin produced by Bacilles thuringiensis BMG1.7, a new strain isolated from soil
Lett. Appl. Microbiol. 32, 1-5
15*SAYARI A, AGREBI N, JAOUA S and GARGOURI Y T (2001)
Biochemical and Molecular characterisation of Staphylococcus simulans lipase
Biochimie 83, 1-9
16*ZOUARI N, BEN SIK ALI S and JAOUA S (2002)
Production of delta-endotoxins by several Bacillus thuringiensis strains exhibiting various entomocidal activities
towards lepidoptera and diptera in gruel and fish-meal media
Enz. Microb. Technol. 31,411-418
17*TOUNSI, S. and JAOUA, S. (2002)
Identification of a promoter for the crystal protein-encoding gene cry1Ia from Bacillus thuringiensis subsp. kurstaki.
FEMS Microbiol. Lett. 208, 215-218
18*ZOUARI N, Achour O and JAOUA S (2002)
Production of delta-endotoxin by Bacillus thuringiensis subsp. kurstaki and overcome of catabolite repression, by
using highly concentrated gruel and fish meal media in 2 and 20 dm3 fermenters
J. Chem. Technol. Biotechnol. 77, 877-882
19*TOUNSI S, ZOUARI N and JAOUA S (2003)
Cloning and study of the expression of a novel cry1Ia-type gene from Bacillus thuringiensis subsp. kurstaki
J. Appl. Microbiol. 94, 1-6
20*TOUNSI S and JAOUA S (2003)
Characterisation of a novel cry2Aa-type gene from Bacillus thuringiensis subsp. kurstaki
Biotechnol. Lett. 25,1219-1223
21*GHRIBI D, ZOUARI N, and JAOUA S (2004)
Improvement of Bioinsecticide Production through mutagenesis of Bacillus thuringiensis subsp. kurstaki by UV and
Nitrous acid affecting metabolic pathways and/or delta-endotoxins synthesis
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J. Appl. Microbiol. 97, 338-346.
22* F. KAMOUN, H. MEJDOUB, H. AOUISSAOUI, J. REINBOLT, A. HAMMAMI AND S. JAOUA (2005)
Purification, Amino Acid Sequence and Characterization of Bacthuricin F4, a new bacteriocin produced by Bacillus
thuringiensis
J. Appl. Microbiol. 98, 881-888
23*D. GHRIBI, N. ZOUARI AND S. JAOUA (2005)
Improvement of bioinsecticides production through Adaptation of Bacillus thuringiensis to heat treatment and NaCl
addition
J. Appl. Microbiol. 98, 823-831
24* L ABDELKEFI MESRATI, S TOUNSI AND S JAOUA (2005)
Characterization of a novel vip3-type gene from Bacillus thuringiensis and evidence of its presence on a large
plasmid .
FEMS Microbiol. Lett. . 244, 353-358
25* F. DRISS, M. KALLASSY-AWAD, N. ZOUARI AND S. JAOUA (2005)
Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki
J. Appl. Microbiol. 99, 945-953
26* HMIDA-SAYARI A, COSTA A, LEONE A, JAOUA S AND R GARGOURI-BOUZID (2005)
Identification of salt stress-induced transcripts in potato leaves by cDNA-AFLP
Mol. Biotechnol. 30, 31-39
27* ABDELKEFI MESRATI L, DAMMAK KARRAY M, TOUNSI S AND JAOUA S (2005)
Construction of a new high copy-number shuttle vector of Bacillus thuringiensis
Lett. Appl. Microbiol. 41, 361-366
28* ABDELKEFI MESRATI L, TOUNSI S, KAMOUN F AND JAOUA S (2005)
Identification of a promoter for the vegetative insecticidal protein-encoding gene vip3LB from Bacillus
thuringiensis.
FEMS Microbiol. Lett. 247, 101-104
29* HMIDA-SAYARI A, GARGOURI-BOUZID R, BIDANI A, JAOUA L, SAVOURÉ A AND JAOUA S (2005)
1
Overexpression of  -pyrroline-5-carboxylate synthetase increases Proline production and confers osmotolerance
in transgenic potato plants
Plant Science, 169, 746-752
30* S. TOUNSI, M. DAMMAK, A. REBAI, AND S. JAOUA (2005)
Response of larval Ephestia kuehniella (Lepidoptera: Pyralidae) to individual Bacillus thuringiensis kurstaki toxins
and toxin mixtures
Biological Control. 35, 27-31
31* R. ZRIBI ZGHAL, S.TOUNSI AND S. JAOUA (2006)
Characterization of another cry4Ba-type gene of Bacillus thuringiensis israelensis and evidence of the synergistic
larvicidal activity of its encoded protein with Cry2A delta-endotoxin of B. thuringiensis kurstaki on Culex pipiens
Biotechnol. And Appl. Biochem. 44, 19-25
32* S. TOUNSI, M. DAMMAK, N. ZOUARI, A. REBAÎ, AND S. JAOUA (2006)
Evidence of the effect of delta-endotoxin ratio in Bacillus thuringiensis crystals on the toxicity against Ephestia
kuehniella
Biological Control. 37, 243-246
33* S. TOUNSI, A. EBN AOUN, M. BLIGHT, A. REBAÎ, S. JAOUA (2006)
Evidence of oral toxicity of Photorhabdus temperata strain K122 against Prays oleae and its improvement by
heterologous expression of Bacillus thuringiensis cry1Aa and cry1Ia genes
J. Inv. Pathol. 91, 131-135
34* S. TOUNSI, N. ZOUARI, D. GHRIBI, A. J’MAL AND S. JAOUA (2006)
Improvement of Bacillus thuringiensis -endotoxins synthesis yields
through acquisition of erythromycin resistance
Biotechnol. Lett. 28, 315-319
35* R ZRIBI ZGHAL AND S JAOUA (2006)
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Evidence of DNA rearrangements in the 128-kilobase pBtoxis plasmid of Bacillus thuringiensis israelensis
Mol. Biotechnol. 33, 191-198
36*TOUNSI SLIM, BLIGHT MARK, JAOUA SAMIR, DE LIMA PIMENTA ANDRÉA (2007)
From insects to human hosts: identification of major genomic differences involved in host adaptation of the
emerging human pathogen Photorhabdus sp.
International Journal of Medical microbiology, 296, 521-530
37*S. ROUIS, M. CHAKROUN, I. SAADAOUI, AND S. JAOUA (2006)
Proteolysis, histopathological effects and immunohistopathological localization of d-endotoxins of Bacillus
thuringiensis subsp. kurstaki in the midgut of Lepidopteran olive tree pathogenic insect Prays oleae
Mol. Biotechnol. 35,1-8
38* M. KALLASSY AWAD, I. SAADAOUI, S. ROUIS, S. TOUNSI AND S. JAOUA (2006)
Differentiation between Bacillus thuringiensis strains by gyrB PCR-Sau3AI fingerprinting
Mol. Biotechnol. 35, 1-7
39*S. KTARI, F. MAHJOUBI, S. JAOUA, A. KARRAY, N. MARTY, S. BEN REDJEB, A. HAMMAMI. (2006) Use of molecular
subtyping methods to investigate two nosocomial outbreaks due to Salmonella Livingstone in Sfax hospital, Tunisia.
Path. Biol. 54, 331-336
40* D. GHRIBI, N. ZOUARI AND S. JAOUA (2007)
Use of sea water as salts source in starch and soya bean based media, for the production of Bacillus thuringiensis
bioinsecticides
Process Biochem. 42, 374-378
41* F. DRISS, A. BAANANNOU, S. ROUIS, I. MASMOUDI, N. ZOUARI AND S. JAOUA (2007)
Effect of the chitin binding domain deletion from Bacillus thuringiensis subsp. kurstaki chitinase Chi255 on its
stability in Escherichia coli
Mol. Biotechnol. 36, 232-237
42* D. GHRIBI, N. ZOUARI, H. TRABELSI AND S. JAOUA (2006)
Improvement of Bacillus thuringiensis delta-endotoxin production by overcome of carbon catabolite repression
through adequate control of aeration
Enz. Microbiol Technol. 40, 614-622
43* S. ROUIS, M. CHAKROUN, AND S. JAOUA (2008)
Comparative study of Bacillus thuringiensis –endotoxin Cry1Aa and Cry1Ac toxin activation, inactivation and in situ
histopathological effect in Ephestia kuehniella (Lepidoptera: Pyralidae)
Mol. Biotechnol. 38, 233-239
44* W JALLOULI, W HAMMAMI, NL ZOUARI, S JAOUA (2008)
Medium optimization for biomass production and morphology variance overcome of Photorhabdus temperata ssp.
temperata strain K122
Process Biochem.43, 1338-1344
45* JUNG WOO-JIN; MABOOD FAZLI; SOULEIMANOV ALFRED; ZHOU XIAOMIN; JAOUA SAMIR; KAMOUN FAKHER;
SMITH DONALD L (2008)
Stability and Antibacterial Activity of Bacteriocins Produced by Bacillus thuringiensis and Bacillus thuringiensis ssp.
kurstaki.
J. Microbiol. Biotechnol. 18, 1836-40.
46* R. ZRIBI ZGHAL, H. TRIGUI, M. BEN ALI AND S. JAOUA (2008)
A single missens mutation in Bacillus thuringiensis subsp. israelensis Cyt1A affects its
cytotoxicity in Escherichia coli
Mol. Biotechnol. 381, 121-127.
47* DAMMAK, MARIAM; TOUNSI, SLIM; ABDELKEFI MESRATI, LOBNA; SCHULTZ, PATRICK; JAOUA, SAMIR (2009)
Restoration of the crystallisation of altered delta-endotoxins Cry1Ac, by the promotion of their in vivo integration
into the Bacillus thuringiensis native crystal
FEMS Microbiology Letters. 292, 268 - 273
48* Imen SAADAOUI, Souad ROUIS, and Samir JAOUA (2009)
New Tunisian Bacillus thuringiensis kurstaki isolate having better toxicity and 
-endotoxin yield
Arch. Microbiol. 191, 341-348
49* Kaïs Jamoussi, Sameh Sellami, Lobna Abdelkefi-Mesrati, Alain Givaudan and Samir Jaoua (2009)
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Heterologous Expression of Bacillus thuringiensis Vegetative Insecticidal Protein-Encoding Gene vip3LB in
Photorhabdus temperata Strain K122 and Oral Toxicity against the Lepidoptera Ephestia kuehniella and Spodoptera
littoralis
Mol. Biotechnol. 43:97-103
50*Fakher Kamoun, Nabil Zouari, Imen Saadaoui, and Samir Jaoua (2009)
Improvement of Bacillus thuringiensis Bacteriocin Production Through Culture Conditions Optimization
Prep. Biochem. & Biotechnol. 39: 400–412
51*Lobna ABDELKEFI-MESRATI, Souad ROUIS, Sameh SELLAMI and Samir JAOUA (2009)
Prays oleae midgut putative receptor of Bacillus thuringiensis Vegetative Insecticidal Protein Vip3LB differs from
that of Cry1Ac toxin
Mol. Biotechnol. 43: 15-19
52*Fakher KAMOUN, Ines BEN FGUIRA, Afef TOUNSI, Lobna ADBELKEFI-MESRATI, Vincent SANCHIS, Didier
LERECLUS and Samir JAOUA (2009)
Generation of Mini-Tn10 transposon insertion mutant library of Bacillus thuringiensis for the investigation of genes
required for its bacteriocin production
FEMS Microbiol. Lett. 294, 141-149
53*Sonia Ktari, Guillaume Arlet, Charlotte Verdet, Samir Jaoua, Amel Kachrid, Saida Ben Redjeb, Fouzia MahjoubiRhimi, Adnane Hammami (2009)
Molecular Epidemiology and Genetic Environment of Acquired blaACC-1 in Salmonella enterica Serotype
Livingstone Causing a Large Nosocomial Outbreak in Tunisia
Microbial Drug Resistance. 15, 279-286
54*Anissa Chouikha, Imene Fodha, Lamjed Bouslama, Mouna Ben Hadj Fredj, Samir Jaoua, Noureddine Boujaafar,
Abdelhalim Trabelsi, and Andrew Duncan Steele. (2009) Emergence and Characterization of Human Rotavirus G9
Strains in Tunisia J. Infect. Dis, 200, 239-243
55* Imen Saadaoui, Nabil Miled and Samir Jaoua (2010)
Evidence of the involvement of E358, A498 and C571 of a new Cry1Ac
 -endotoxin of Bacillus thuringiensis in its
high insecticidal activity against Ephestia kuehniella
Mol. Biotechnol, 45. 65-70
56*Wafa Jallouli, Nabil Zouari and Samir Jaoua (2010)
Involvement of oxidative stress and growth at high cell density in the viable but nonculturable state of
Photorhabdus temperata ssp. temperata strain K122
Process Biochemistry, 45, 706–713
57*Mariam Dammak, Slim Tounsi, Souad Rouis and Samir Jaoua (2010)
Comparative study of Bacillus thuringiensis Cry1Ia and Cry1Aa delta-endotoxins : activation process and toxicity
against Prays oleae
J. Inv. Pathol.104, 39-43
58* Imen Saadaoui, Roda Al-Thani, Fatma Al-Saadi, Lobna Abdelkafi Mesrati, Najeh, Belghith Ben Hassin, Patrick
Schultz, Souad Rouis and Samir Jaoua (2010)
Characterization of Tunisian Bacillus thuringiensis Strains with Abundance of kurstaki Subspecies Harbouring
Insecticidal Activities Against the Lepidopteran Insect Ephestia kuehniella
Curr Microbiol. 61, 541-548
59* Olfa KILANI-FEKI, Oussama KHIARI, Gérald CULIOLI, Annick ORTELO-MAGNE, Nabil ZOUARI, Yves BLACHE and
Samir JAOUA (2010)
Antifungal activities of an endophytic Pseudomonas fluorescens strain Pf1TZ harbouring genes from pyoluteorin
and phenazine clusters
Biotechnol. Lett. 32, 1279-1285
60* Trabelsi A, Fodha I, Chouikha A, Ben Hadj Fredj M, Mastouri M, Abdelaziz AB, Sfar T, Essoussi AS, Jaoua S,
Steele AD (2010) Rotavirus strain diversity in the Centre Coast of Tunisia from 2000 through 2003.
J. Infect. Dis. 202, 252-257
61* Anis Ben HSOUNA, Mohamed TRIGUI, Gérald CULIOLI, Yves BLACHE and Samir JAOUA ( March 2011)
Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure elucidation and antioxidative capacity
Food Chem. 125, 193-200
62*Fatma Driss, Souad Rouis, Hichem Azzouz, Slim Tounsi, Nabil Zouari, Samir Jaoua (2011)
11
Integration of a Recombinant Chitinase into Bacillus thuringiensis Parasporal Insecticidal Crystal.
Curr. Microbiol. 62, 281-288.
63*Saoussen Ben Khedher, Nabil Zouari, Nadia Messaddeq, Patrick Schultz and Samir Jaoua (2011)
Overproduction of Delta-Endotoxins by Sporeless Bacillusthuringiensis Mutants Obtained by Nitrous Acid
Mutagenesis
Curr. Microbiol. 62, 38-43
64* Lobna Abdelkefi-Mesrati, Hanen Boukedi, Mariam Dammak-Karray, Tahya Sellami-Boudawara, Samir Jaoua
and Slim Tounsi (2011)
Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding
proteins in the midgut of Spodoptera littoralis
J. Inv. Pathol. 106, 250–254
65*Fatma DRISS, Slim TOUNSI and Samir JAOUA (2011)
Relationship between plasmid loss and gene expression in Bacillus thuringiensis
Curr. Microbiol. 62:1287-1293
66* Olfa Kilani-Feki, Gérald Culioli, Annick Ortalo-Magné, Nabil Zouari, Yves Blache and Samir Jaoua (2011)
Environmental Burkholderia cepacia strain CS5 acting by two analogous alkyl-quinolones and a didecyl-phthalate
against a broad spectrum of phytopathogens fungi
Curr. Microbiol. 62, 1490–1495
67*Ben Khedher, S., Jaoua, S. and Zouari, N. (2011).
Improvement of Bioinsecticides Production by Sporeless Bacillus thuringiensis strains in Response to Various
Stresses in Low Cost Medium. Curr. Microbiol. 62 : 1467-1477
68* Anis BEN HSOUNA, Mohamed TRIGUI, Riadh BEN MANSOUR, Raoudha MEZGHANI JARRAYA, Mohamed DAMAK
and Samir JAOUA (2011)
Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with
preservative effects against Listeria inoculated in minced beef meat
Int. J. Food Microbiol. 148, 66–72
69*Saoussen BEN KHEDHER, Amel KAMOUN, Samir JAOUA and Nabil ZOUARI. (2011).
Improvement of Bacillus thuringiensis bioinsecticides production by sporeless and sporulating strains using
response surface methodology .
New Biotechnol. 28, 705-712
70* Fakher Kamoun, Ines Ben Fguira, Najeh Belguith Ben Hassen, Hafedh Mejdoub, Didier Lereclus, Samir Jaoua
(2011)
Purification and characterization of a new Bacillus thuringiensis bacteriocin active against Listeria monocytogenes,
Bacillus cereus and Agrobacterium tumefaciens
Appl. Biochem. Biotechnol.165, 300-314
71* Lobna Abdelkefi-Mesrati, Hanen Boukedi, Maissa Chakroun, Fakher Kamoun, Hichem Azzouz, Slim Tounsi,
Souad Rouis and Samir Jaoua (2011)
Investigation of the steps involved in the difference of susceptibility of Ephestia kuehniella and Spodoptera littoralis
to the Bacillus thuringiensis Vip3Aa16 toxin
J. Inv. Pathol. 107, 198-201
72* Sameh Sellami, Kaïs Jamoussi, Emna Dabbeche and Samir Jaoua (2011)
Increase of the Bacillus thuringiensis secreted toxicity against lepidopteron larvae by homologous expression of the
vip3LB gene during sporulation stage
Curr. Microbiol.63, 289-294 Sept
73*Jallouli W, Jaoua S, Zouari N (2011)
Overcoming the production limitations of Photorhabdus temperata ssp. temperata strain K122 bioinsecticides in
low-cost medium.
Bioprocess Biosyst Eng.34, 1039-1047
74* Olfa Kilani-Feki and Samir Jaoua (2011)
Biological control of Botrytis cinerea using an antagonictic and endophytic Burkholderia cepacia (Cs5) for the vine
plantlets protection
Canad. J Microbiol. 57, 896-901
12
75*Anis Ben Hsouna, Mongi Saoudi, Mohamed Trigui, Kamel Jamoussi, Tahia Boudawara, Samir Jaoua, Abdelfattah
El Feki (2011)
Characterization of bioactive compounds and ameliorative effects of Ceratonia siliqualeaf extract against CCl4
induced hepatic oxidative damage and renal failure in rats
Food Chem. Toxicol. 49, 3183-3191
76*Mariam Dammak, Samir Jaoua, Slim Tounsi (2011)
Construction of a Bacillus thuringiensis genetically-engineered strain harbouring the secreted Cry1Ia deltaendotoxin in its crystal
Biotechnol. Lett..33,2367-2372
77* Mongi Saoudi, Anis Ben Hsouna, Mohamed Trigui, Kamel Jamoussi, Samir Jaoua and Abdelfatteh Feki (2011)
Differential oxidative stress responses to methanol in intraperitoneally exposed rats: Ameliorative effects of
Opuntia vulgaris fruit extract.
In press in Toxicol. Indust. Health
78*Imen Blibech, Mohieddine Ksantini, Ikbal Chaieb, Brahim Jlassi, Ali Rhoumaa, Samir Jaoua, Sami Aifa (2011)
Isolation of entomopathogenic Bacillus from a biodynamic olive farm and their pathogenicity to lepidopteran and
coleopteran insect pests
Crop Protection; 31, 72-77
79* Olfa Kilani-Feki, Imen Zouari, Gérald Culioli, Annick Ortalo-Magné, Nabil Zouari Yves Blache and Samir Jaoua
(2012)
Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of aBurkholderia cepacia
strains collection
World J. Microbiol. Biotechnol.,28, 275 - 281.
80* Mariam Dammak, Mamdouh Ben Ali, Samir Jaoua, Slim Tounsi (2012)
Amino acids Y229 and F603 are involved in Bacillus thuringiensis Cry1Ac delta-endotoxin stability and toxicity
FEMS Microbiol. Lett. 329, 54-60
81*Abdelhi Dihazi, Ph.D.; Fatima Jaiti; Wafa Taktak; Olfa kilani Feki; Samir Jaoua; Azeddine Driouich; Mohamed
Baaziz; Fouad Daayf; Mohammed Amine Serghini (2012)
Use of two bacteria for biological control of bayoud disease caused by Fusarium oxysporum in date palm (Phoenix
dactylifera L) seedlings
Plant Physiol. Biochem 55, 7 – 15
82*Wafa Jallouli, Samir Jaoua and Nabil Zouari (2012)
Improvement of Photorhabdus temperata strain K122 bioinsecticide production by batch and fed-batch
fermentations optimization.
Bioprocess and Biosys. Eng..In press
83*Sellami S, Zghal T, Cherif M, Zalila-Kolsi I, Jaoua S, Jamoussi K. (2012)
Screening and identification of a Bacillus thuringiensis strain S1/4 with large and efficient insecticidal activities.
J. Basic Microbiol. In press
84*Olfa Kilani-Feki, Fakher Frikha, Imen Zouari and Samir Jaoua (2012)
Heterologous expression and secretion of an antifungal Bacillus subtilis chitosanase (CSNV26) in Escherichia coli
Bioprocess and Biosystems Engineering. In Press
13- COLLABORATIVE PROJECTS: with laboratories from Tunisia, Qatar, Morocco, France,
Portugal, Canada, Spain, Italy, Germany, Algeria, Libanon, ..: Microbial Genetics, Microbial
metabolites, biopesticides, Biotechnology…
14- SCIENTIFIC COOPERATION ABROAD
-Dr Didier Lereclus, INRA, Versaille, France
-Pr Hedi Hitakura, Niigata Univ, Japon
-Pr Jean Charles Du Côté, Canada
-Pr Jean Noêl Bomarre, IN*RA, Montpellier, France
-Dr Mark Blight, Univ Paris XI, Orsay, France
13
-Bruno Figadère, Paris, France
-Pr Claude Garnier, Fac Pharmacie, Montpellier, France
-Pr Abdelaziz Soukri, Fac Sc, Casablanca, Maroc
-Pr Ismail Hadhrami, Marrakech, Maroc
-Dr Mireille Kallassy, Beyrouth, Liban
-Dr Alain Givaudain, Montpellier, France
-Pr Yves Blaches, Toulon France
-Pr Quirici Mighelli, Sassari, Italy
-Dr Roda Al Thani, Doha , Qatar
-Dr Patrick Schultz, Institut de Biotechnology, Illkirch, France
-Pr Nelson Simoes, Panta delgada, Portugal
-Pr Juan Ferre, Valencia Spain
-Dr Roda Al-Thani, Doha, Qatar
15-EXPERIENCE IN PEER REVIEWING
Research/cooperative projects:
-Tunisian Ministy of higher education, scientific research and technology, projects and laboratories
evaluation
-International organisations : AUF, IAAE, UNEP….
Scientific articles:
J. Gen Microbiol. , FEMS Microbiol. Lett., Appl. Biochem. Biotechnol., Enz. Microb. Technol., Lett. Appl.
Microbiol., J. Appl. Microbiol, Mol. Biotechnol., ...
16-FINANCIAL SUPPORT:
From Tunisian Ministry of Higher Education and Scientific research,
International Center for Genetic Engineering and Biotechnology, TWAS, ISESCO, AUF, NEPAD, QNRF,
Qatar University,...
2011: 1ST year of a new NPRP project [LPI] and 2nd year of another NPRP project [Co-PI]
17-INTERNATIONAL PATENT
JAOUA S, SCHUPP T and NEFF S ( Nov 1997)
United States Patent N° : 5,686,295 ; Appl. N° : 276752
Process for Genetic Manipulation of Myxobacteria
Assignee : Novartis Finance Corporation (New York, NK)
http://www.patents.com/us-5686295.html
18-TUNISIAN NATIONAL PATENTS
1*JAOUA S, ZOUARI N, TOUNSI S, BELGUITH-BEN HASSEN N and ELLOUZ R (2000)
Tunisian Patent Appl. N° : 17393, INNORPI
Mise au point d’une collection de 75 souches de Bacillus thuringiensis productrices de Bioinsecticides à large spectre d’activités
et de bactériocines
Assignee : Centre de Biotechnologie de Sfax, Tunisia
2*ZOUARI N and JAOUA S (2000)
Tunisian Patent Appl. N° :410/00, INNORPI
Mise au point de milieux de culture à base de gruau et de farine de poisson pour la production de bioinsecticides à large spectre
d’activités, par des souches de Bacillus thuringiensis
Assignee : Centre de Biotechnologie de Sfax, Tunisia
3* SLIM TOUNSI, MARIAM DAMMAK, NABIL ZOUARI ET SAMIR JAOUA (2004)
14
Mise au point d’une nouvelle méthode de co-cristallisation de protéines et particulièrement la delta-endotoxine Cry1Ia de
Bacillus thuringiensis avec les autres delta-endotoxines qui forment le cristal de Bacillus thuringiensis.
Tunisian Patent Appl, INNORPI, SN04131
4* SAMIR JAOUA, NABIL ZOUARI, SLIM TOUNSI, SOUAD ROUIS, IMENE SAADAOUI, FAKHER KAMOUN, LOBNA ABDELKEFIMESRATI, DHOUHA GHRIBI-AYDI ET NAJEH BELGUITH-BEN HASSEN (2004)
Isolement et Mise en oeuvre d’une souche de Bacillus thuringiensis productrice d’un nouveau Bioinsecticide actif sur les larves
d’insectes appartenant à la famille des lépidoptères
Tunisian Patent Appl. SN4263, INNORPI
5* SLIM TOUNSI, ANIS J'MAL, NABIL ZOUARI & SAMIR JAOUA (2005)
Mise au point d'une Méthode Génétique d'Amélioration de la Production de Bioinsecticides de Bacillus thuringiensis
Assignee : Centre de Biotechnologie de Sfax, Tunisia
Tunisian Patent Appl. SN5041, INNORPI
6* DHOUHA GHRIBI, NABIL ZOUARI & SAMIR JAOUA (2005)
Utilisation de l’eau de mer comme source de sels dans les milieux de production de delta-endotoxines (bioinsecticides) de
Bacillus thuringiensis
Tunisian Patent Appl, 5040 INNORPI
7*AÏDA HMIDA-SAYARI, AMIRA BIDANI, LEÏLA JAOUA, RADHOUANE ELLOUZ, SAMIR JAOUA ET RADHIA GARGOURI-BOUZID
(2005)
Construction de Plantes de pomme de terre transgéniques exprimant le gène delta-1 pyrroline-5-carboxylate synthétase
conférant la tolérance à la salinité
Tunisian Patent Appl, 5093 INNORPI
8*SAMIR JAOUA, Raïda ZRIBI ZGHAL, Najeh BELGUITH-BEN HASSEN, Mohamed Jemaa et Hichem AZZOUZ (2008)
Un nouveau bioinsecticide d’une souche tunisienne (BUPM98) de Bacillus thuringiensis israelensis constitué de nouvelles deltaendotoxines fortement actives sur les larves d’insectes appartenant à la famille des diptères et vecteurs de maladies.
th
Tunisian Patent Appl, SN08193 INNORPI April 30 2008
9* MOHAMED TRIGUI, ANIS BEN HSOUNA, KARAMA ZOUARI BOUASSIDA, GERALD CULIOLI, YVES BLACHE et SAMIR JAOUA
(2009)
Isolement et caractérisation des activités biologiques de deux nouvelles molécules biologiquement actives ainsi que d’extraits
organiques à partir de la plante Lawsonia inermis LBPes03
Tunisian Patent Appl, INNORPI September 2009
10* Olfa KILANI-FEKI, Samir JAOUA et Imen ZOUARI (2009)
Une nouvelle chitosanase CSNV26, produite par une souche isolée de Bacillus subtilis V26, thermostable et capable d’être
secrétée en abondance dans le surnageant de culture d’Escherichia coli exprimant cette enzyme d’une manière hétérologue»
Tunisian Patent Appl, INNORPI June 2009
19-INTERNATIONAL CONFERENCES AND MEETINGS
-Samir Jaoua has been invited to make a big number of oral presentations, dealing with his research activities at
several international Conferences as well as seminars in Universities and Research Institutions in US,,Europe, Japan,
Canada, Qatar, Middle East, Thailand, India, Africa, ..
The last International Conference attended: Keynote speaker at the Congress of the AUF (Agence Universitaire de la
Francophonie –BioVeg, France), 26-30 September 2010, Cluj, Romania
Oral presentation entitled: “Antifungal activities of an endophytic Pseudomonas fluorescens strain Pf1TZ harbouring
pyoluteorin and phenazine clusters”
20-ORGANIZATION OF INTERNATIONAL CONFERENCES AND MEETINGS
-2008: Samir Jaoua has been the chairman of Biotechnology conference organised by the Arab, Science and
Technology foundation, in Fez (Morocco) October 2009
-2008: Samir Jaoua has prepared the scientific programme of the International symposium on biotechnology, 4-8
May 2008 Sfax, Tunisia
21-LIST OF REFEREES
*Pr Daniel THOMAS
Director of the Laboratory of Enzymatic Technology, University of Technology of Compiègne, Centre de Recherche
Royallieu
60206 Compiègne cedex France
*Pr Marc Van MONTAGU
Director of the Flemish Institutes of Plant Biotechnology
15
Laboratorium Genetika. K L Ledeganckstraate 35. 9000 Gent. Belgium
E. mail : [email protected], Tel : 3292645205
*Pr Radhouane ELLOUZ
Ex-Director General of the Centre of Biotechnology of Sfax.
P.O.Box. : 1177. 3038. Sfax. Tunisia. Tel/Fax: 21674874446,
E.mail : [email protected]
22-LANGUAGES
Arabic, French and English: well written and spoken; German: spoken