CV - Universitäts KrebsCentrum Dresden

CV - Universitäts KrebsCentrum Dresden

Epigenetic regulation and genomic engineering
Research topic(s):
1. Transcriptional regulation, chromatin, epigenetic regulation
2. histone methylation, histone variants
3. DNA chemistry, recombination, genetic engineering
4. secondary metabolism, molecular evolution
Group leader(s):
Prof. Francis Stewart
Professur für Genomik
Tatzberg 47/49
01307 Dresden
Ph.:
+49-351-463-40130
Fax:
+49 -351-463-40143
E-mail:
[email protected]
Homepage: http://www.biotec.tu-dresden.de/research/stewart/group-page/
Education:
1977 - 1980:
BSc (Hons 1st), University of New South Wales, Sydney, Australia
1981 - 1985:
PhD, University of New South Wales, Sydney, Australia
Training:
07/85 - 12/90
Post-doc and visiting scientist, German Cancer Research Centre (DKFZ),
Heidelberg
Clinical Experience:
Professional Appointments:
01/91 - 07/01:
Group Leader Gene Expression Program, European Molecular Biology
Laboratories (EMBL), Heidelberg
2001 – 2004
Guest Group Leader, Max-Planck-Institute for Cell Biology and genetics (MPI
– CBG), Dresden
08/01 - present: Professor, Genomics, BioinnovationsZentrum, Dresden University of
Technology
Publications:
150 scientific publications (125 primary research publications, 25 reviews
& book chapters, Hirsch-Index: 58)
Funding:
DFG, EU, BMBF, GIF, SMWK, Fresenius
Award:
ISTT Preis 2010, EMBO Fellow 2007
Journals:
no editor position, but regular reviews
Grant review:
DFG, European Commission, 7th Framework, European Research
Council, European Science Foundation (ESF), Wellcome Trust
Societies:
DKFZ Alumni, EMBL Alumni
Group members:
Scientists:
PhD students:
20
Dr. F. Groß, Dr. A. Kranz, Dr. H. Hofemeister, Dr. S. Subramaniam, Dr.
Jun Fu, Dr. G. Ciotta
K. Bernhardt, R. Choudhury, A. Gupta, S. Singh, D.C. Torres, S. Tsurkan,
J. Yin
Internal collaborators:
Dr. Konstantinos Anastassiadis, Stem Cell Group, Biotechnology Center, TU Dresden.
Dr. Frank Buchholz, Functional Genomics in Mammalian Cells, MPI-CBG, Dresden
Prof. Wieland Huttner, Cell biological basis of mammalian neurogenesis, MPI-CBG, Dresden
Prof. Anthony Hyman, Microtubules and cell division, MPI-CBG, Dresden
Prof. Rolf Jessberger, Institute of Physiological Chemistry, MTZ, TU Dresden
Dr. Mihail Sarov, Transgenomics Facility, MPI-CBG, Dresden
Dr. Andrej Schevchenko, Mass spectrometry in functional characterization of biological
molecules, MPI-CBG, Dresden
Dr. med. Christian Thiede, Medizinische Klinik und Poliklinik I, UKD, TU Dresden
Dr Attilla Toth, Mammalian Meiosis Group, MTZ, TU Dresden
External collaborators:
Prof. Rein Aasland, Department of Molecular Biology, University Bergen, Norwegen
Prof. Philip Avner, Institut Pasteur, Paris, Frankreich
Dr. Andreas Beyer, Universität KÖln
Prof. Oliver Brüstle, Institute of Reconstructive Neurobiology, Universität Bonn
Dr. Pamela Cowin, Cell Biology, Medical Science Building, New York University, USA
Prof. Karl Ekwall, Department of Biosciences and Nutrition, Karolinska Institute, Schweden
Prof. Frank Grosveld, Dept. of Cell Biology, Erasmus MC, Rotterdam, Niederlande
Dr. Edith Heard, Developmental Biology and Genetics, Institute Curie, Paris, Frankreich
Prof. Matthias Mann, Department of Proteomics and Signal Transduction, MPI Biochemistry,
Martinsried
Prof. Fritz Melchers, MPI for Infection Biology, Berlin
Prof. Rolf Müller, Department of Pharmaceutical Biotechnology, Universität des Saarlandes,
Saarbrücken
Prof. Karla Neugebauer, Yale University, New Haven, USA.
Prof. Udo Oppermann, Structural Genomics Consortium, Oxford University, UK
Prof. Nadia Rosenthal, Australian Regenerative Medicine Institute, Monash University,
Melbourne, Australia.
Prof. Erik Schäfer, Universität Tubingen
Prof. Pete Shaw, Biomedical Sciences, University of Nottingham, UK
Dr. William Skarnes, Wellcome Trust Sanger Institute, Hinxton, UK
Prof. Austin Smith, Wellcome Trust Stem Cell Institute, Cambridge University, UK
Prof. Henk Stunnenberg, Molecular Biology, Radboud University Nijmegen, Niederlande.
Dr. Michiel Vermeulen, Radboud University Nijmegen, Niederlande.
Prof. Harald von Melchner, Med. Klinik II, Hämatologie/Onkologie, Goethe-Universität Frankfurt
Prof. Wolfgang Wurst, Institute of Developmental Genetics, Helmholtz Zentrum München,
Neuherberg/München
Dr. Youming Zhang, Shandong University, Shandong Provence, China.
Participation in collaborative research projects (local, national, international):
local:
SMWK, EFRE: Ausbau der Technologieplattformen des BIOTEC in den Bereichen
bioinformatische Services, Fish Facility, genetische Services, Mikroskopie/ Imaging
national:
DFG, SPP 1356: Robust model systems for analyzing the reprogramming of somatic cells into
pluripotent cells.
DFG, SPP 1463: Epigenetic regulation of normal hematopoiesis and its dysregulation in myeloid
neoplasia.
BMBF, DiGTOP: Disease Genes to Protein Pathways
international:
EU, FP7: SyBOSS, EUCOMMtools
List of 10 most important publications 2005–2014:
1. Denissov S, Hofemeister H, Marks H, Kranz A, Ciotta G, Singh S, Anastassiadis K,
Stunnenberg HG and Stewart AF. (2014) Mll2 is required for H3K4 trimethylation of
bivalent promoters in ES cells whereas Mll1 is redundant. Development, 141, 526-37.
2. Bledau A, Schmidt K, Neumann K, Hill U, Ciotta G, Gupta A, Torres DC, Fu J, Kranz A,
Stewart AF and Anastassiadis K. (2014) The H3K4 methyltransferase Setd1a is first
required at the epiblast stage, whereas Setd1b becomes essential after gastrulation.
Development, 141, 1022-35.
3. Rostovskaya M, Fu J, Obst M, Baer I, Weidlich S, Wang H, Smith AJH, Anastassiadis K
and Stewart AF. Transposon mediated BAC transgenesis in human ES cells (2012)
Nucleic Acids Res. 40, e150.
4. Bird AW, Erler A, Fu J, Hériché J-K, Maresca M, Zhang Y, Hyman AA and Stewart AF.
(2011) High efficiency counterselection recombineering for site-directed mutagenesis in
bacterial artificial chromosomes. Nature Methods, 9, 103-109.
5. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Cox T, Jackson D,
Severin J, Biggs P, Thomas M, Mujica A, Harrow J, Fu J, Nefedov M, de Jong P,
Stewart AF and Bradley A. (2011) A conditional knockout resource for genome-wide
analysis of mouse gene function. Nature 474, 337-42.
6. Hofemeister H, Ciotta G, Fu J, Seibert PM, Schulz A, Maresca M, Sarov M,
Anastassiadis K and Stewart AF (2011). Recombineering, transfection, Western, IP and
ChIP methods for protein tagging via gene targeting or BAC transgenesis. Methods 53,
437-52.
7. Andreu-Vieyra CV, Chen R, Agno J, Glaser S, Anastassiadis K, Stewart AF and Matzuk
MM. (2010) Mll2 is required in oocytes for bulk histone 3 lysine 4 trimethylation and
global transcriptional silencing. PLoS Biology, 17, 8(8).
8. Anastassiadis K, Fu J, Patsch C, Hu S, Weidlich S, Duerschke K, Buchholz F, Edenhofer
F and Stewart AF (2009). Dre recombinase, like Cre, is a highly efficient site-specific
recombinase in E. coli, mammalian cells and mice. Dis Model Mech 2: 508-15.
9. Glaser S, Lubitz S, Loveland KL, Ohbo K, Robb L, Schwenk F, Seibler J, Roellig D,
Kranz A, Anastassiadis K and Stewart AF (2009). The histone 3 lysine 4
methyltransferase, Mll2, is only required briefly in development and spermatogenesis.
Epigenetics Chromatin 2: 5.
10. Glaser S, Anastassiadis K and Stewart AF (2005). Current issues in mouse genome
engineering. Nature Genetics 37: 1187-93.