docHigh-Technology, Biomedical Imaging Core Facility
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High-Technology, Biomedical Imaging Core Facility
contact information Locations Scientific Director Site Managers Frank Lafont 03 20 87 11 36 [email protected] Administrative Staff Lille 1 Campus Lille 1 Campus Christian Slomianny Dominique Legrand 03 20 33 70 63 [email protected] Hafida Kameli 1 rue du professeur Calmette 59021 Lille Cedex Teaching Hospital Campus - Light, Atomic Force & Electron Microscopy Flow Cytometry, High-Content Screening Teaching Hospital Campus Sophie Crespin Valérie Mitchell Murielle Verghote 03 20 44 54 85 [email protected] Pasteur Lille Campus Frank Lafont 03 20 87 11 36 [email protected] 1 km Pasteur Lille Campus Quality Management N227 Pasteur Lille Campus Villeneuve-d'Ascq A25 Frédéric Lefèbvre Abdelhafid Arbaoui Electron Microscopy Light Microscopy Computer Systems, Network & Telecom A27 High-Technology, Biomedical Imaging Core Facility A1 A23 Romain Desruelle Inter-Site Coordinator Inter-Site Coordinator Laurent Héliot Christian Slomianny Lille 1 Campus High-Content Screening Lille 1 Campus Loïc Brunet Laurent Héliot Anne-Sophie Lacoste Inter-Site Coordinators Elodie Richard Christian Slomianny Priscille Brodin Christian Slomianny Corentin Spriet Benoît Déprez Pasteur Lille Campus Nicolas Barois Teaching Hospital Campus Frank Lafont Gaspard Deloison Delphine Lacorre Frank Lafont Elisabeth Werkmeister Flow Cytometry Pasteur Lille Campus Inter-Site Coordinator Alain Baulard Thierry Idziorek Antonino Bongiovanni Frank Lafont Pasteur Lille Campus Simone Bovio Yann Ciczora Sébastien Janel Frank Lafont - Flow Cytometry - Light & Electron Microscopy Flow Cytometry supervisory institutions: See better Priscille Brodin Lille 1 Campus Cyril Couturier Agnès Denys Nathalie Deboosere Teaching Hospital Campus Thierry Idziorek Nathalie Jouy Inter-Site Coordinator Institut de Recherche sur le Cancer (IRCL) Bd. du Pr Jules Leclercq 59045 Lille Cedex UFR de Biologie Cité Scientifique Bâtiment SN3 Bâtiment C9 59655 Villeneuve d’Ascq Cedex Corentin Le Nezet Gaspard Deloison Atomic Force Microscopy Lille 1 Campus - Light Microscopy Laurent Héliot Pasteur Lille Campus Antonino Bongiovanni Lille 1 Campus Faculté de Médecine Henri Warembourg Pôle Recherche - 4ème étage Est 1 place de Verdun 59045 Lille Cedex Mariano Gonzalez Pisfil Valérie Mitchell Meryem Tardivel Laurent Héliot Teaching Hospital Campus Emeline Machez Pasteur Lille Campus Benoît Déprez supporting authorities and associations: Adrien Herlédan Delphine Lacorre Frank Lafont Valérie Landry Florence Leroux Virginie Pottiez Hélène Bauderlique Alexandre Vandeputte Christelle Faveeuw Elisabeth Werkmeister approved for research tax credit in france (cir) An up-to-date version is available at: www.bicel.org Version: 2014.6 Understand further Add value Light microscopy faciLity atomic force microscopy faciLity • Multiphoton microscopy 5 core facilities to meet your research needs Created in January 2010, the BioImaging Center Lille-Nord de France (BICeL) consists, within a transversal operation set-up, in 5 core facilities gathering atomic force, photonic and electron microscopy as well as flow cytometry and high-content screening facilities of Lille 1 University - Sciences and Technologies -, Lille 2 Medical School and Lille Pasteur Institute campuses. Approved by the national Scientific Interest Group for Infrastructures in Biology, Health and Agronomy (GIS-IBiSA), BICeL’s infrastructures and expertise are available to scientists in both academia and industry. Developments are more particularly focused on high-resolution analyses and the tracking of dynamic molecular interactions. • Nanoscale topography imaging • Upright & inverted, conventional & fluorescence microscopes (Leica, Nikon, Olympus, Zeiss) - BSL-1&2* • Fluorescence Recovery After Photobleaching (FRAP) • Förster Resonance Energy Transfer (FRET) • Quantitative imaging of samples physical properties • Elasticity measurements & stiffness tomography by indentation • Stereo-microscopes for aquatic animals imaging • Fluorescence lifetime measurements (FLIM and SLIM) • Cell receptors mapping with functionalized AFM tips • Upright Apotome (Zeiss) with deconvolution module • Fluorescence (Cross-)Correlation Spectroscopy (FCS and FCCS) • Molecular and cell adhesion measurements • Laser microdissection (Leica) • Total Internal Reflection microscopy (TIRF) • Video-microscopes (Leica, Zeiss) - BSL-1&2*: ion imaging, deconvolution / 3D reconstruction, infra-red and patch-clamp • Super-resolution microscopy (PALM/STORM, SIM, STED) • Small animal in vivo imaging • Confocal microscopes (Leica, Nikon, Zeiss): white light laser (R&D), spinning disk (R&D) Applications • Multiphoton microscopes (Leica): SP5 (R&D) - BSL-1*, SP8 IVM - BSL-2* • (Single-) molecule localization, tracking & activity assessments • Super-resolution microscopes (Nikon/Andor, Zeiss & Abberior Instruments): PALM, SR-SIM (R&D), STED (R&D) • Dynamic study of cell organelle distribution • Functional dynamics of molecular interactions • Multimode whole body imagers (Perkin Elmer and Bruker): in vitro, ex vivo & in vivo bioluminescence, fluorescence and/or X-rays - BSL-1, 2 & 3* • Membrane trafficking • Cell survival • Intracellular calcium flux • Motion detection in constant force mode *Biosafety Level 1, 2 & 3 Laboratories B io I magIng c enter L Ille Methods Equipment • Nanomanipulation of biological objects Applications • Membranes structure and elasticity in live cells • Adhesion & elasticity of spores, bacteria, biofilms & parasites • Membrames viscoelastic properties • Cell mapping of bacterial receptors • Biophysical characterization of individual molecules or complexes interactions • AFM / Fluorescence combination on a nanometer scale Equipment • Bruker BioScope Catalyst coupled with Zeiss PALM ELYRA P.1 • Bruker BioScope Catalyst coupled with Zeiss fluorescence TIRF - LNSB2* • Bruker MultiMode 8 • Bruker FastScan Bio • JPK NanoWizard3 with CellHesion 2000 stage on a Zeiss AxioObserver.Z1 • JPK BioMat on a Zeiss AxioImager.M2m • JPK NanoWizard3 Ultra coupled with an Abberior Instruments RESOLFT system • Electrophysiology • Contraction amplitude and frequency measurements in cardiomyocytes eLectron microscopy faciLity fLow cytometry faciLity high-content screening faciLity Sample Preparation Equipment Analyzers Equipment • Balzer SCD040 metalizer • Beckman Coulter CyAn ADP • Leica EM HPM100 high-pressure fast freezing system, Leica Reichert AFS and EM AFS2 freeze substitution, low temperature inclusion and UV polymerization systems equipped with Leica EM FSP processor • Beckman Coulter Cell Lab Quanta • Perkin Elmer Opera QEHS automated 96-, 384- & 1536-well microplate reader - LNSB3* Cytomat 2C plate incubator 4 lasers (405 nm, 488 nm, 561 nm, 640 nm) 3 High QE CCD cameras Objectives: 10x_Air, 20x_Air, 20x_Water & 60x_Water The broad range of application fields includes metabolic diseases (diabetes, obesity and Alzheimer disease), cancer, neurosciences, inflammation, immunity and infectious diseases. *Biosafety Level 2 Laboratory Methods • Becton Dickinson LSRFortessa • Becton Dickinson FACSCalibur • Leica Ultracut R ultramicrotome • Leica Ultracut UCT / EM FCS chamber, and Leica EM UC7 / FC7 chamber cryo-ultramicrotomes Methods • Gatan EM 914 cryo-transfer system • Inclusion for ultrastructural morphology & immunostaining Imaging Equipment • Semi- and ultra-thin sections at room temperature • Hitachi H7500 TEM (120 kV) equipped with a Hamamatsu Photonics ORCA camera (1 Mpixel) • Ultra-thin cryosections • Hitachi H600 TEM (100 kV) • Negative staining • Jeol JEM 2100 (200kV) cryo-tomo-analytical TEM equipped with STEM-HAADF, Bruker Quantax 400 EDS, Gatan 964 Quantum SE 4K GIF, and Ultrascan 4000 (16 Mpixel) & Orius SC200D (4 Mpixel) cameras • Immunohisto- & cytochemistry before or after inclusion • Correlative light, AFM & electron microscopy • Histo- and cytochemistry • EDX and EELS microanalyses • 3D imaging • Zeiss Merlin Compact VP SEM equipped with Gatan 3View module • Applications • Ion channels, intracellular trafficking • Nuclear receptors • Neurobiology and neurodegenerative diseases Cell Sorters • Becton Dickinson FACSAria III Methods • Becton Dickinson FACSAria • Immunostaining Imaging Flow Cytometer • Functional staining with fluorescent probes • Amnis ImageStreamx Mark II • Nucleic acid staining • GE Healthcare Life Sciences IN Cell Analyzer 6000 automated 96-, 384- & 1536-well microplate reader - LNSB2* Incubation chamber (37°C) 4 lasers (405nm, 488nm, 561nm, 640nm) & LED (transmitted light) sCMOS 5.5 MP camera Objectives: 4x_Air, 10x_Air, 20x_Air & 63x_Air Methods • Confocal fluorescence microscopy (Opera : spinning disk, IN Cell: line scan) • Wide-field fluorescence imaging (IN Cell) Applications • Cellular, microbiological and viral studies • Rare events analysis, stem cell studies • Multiphenotyping • Transmitted light imaging (IN Cell) • 2D & 3D (Z-stack) acquisitions, kinetics • Data storage and management: Columbus • Image analysis: Acapella, Columbus, IN Cell Investigator • Transfection rate assessment • Cell proliferation and cell death studies • Functional studies • Absolute count and assessment of fluorescence concentrations • Adenovirus, bacterial infection of cells • Simple to high-throughput, 2- to 4-way cell sorting in tubes, slides as well as 96- & 384-well microplates • Mitochondria, lipoproteins, cytoskeleton, organelle stress • Imaging flow cytometry • Biomaterials, nanotechnologies, nanoparticles *Biosafety Level 2 & 3 Laboratories An original microscopy-based screening core facility for biomedical applications (therapeutic active compounds, vaccines, multiparametric screening of gene expression silencing by cell-based assay, ...) is emerging with the support of the French government-sponsored “Investments for the future” program: 2011 Equipment of Excellence “ImaginEx BioMed”, supplemented by an EU-Region-State funding (European Economical and Regional Development Fund). Applications • High-throughput screening of fluorescent samples microplates Opera troughput : 10, 000 points - 50, 000 images a day • Cellular microbiology with biosafety level 2 & 3 pathogens • 2D, 3D & 2D+Time colocalization of intracellular events • Real-time live cell imaging and acquisitions in fixed samples